共发表学术论文超过180篇，H指数：98，总引用逾35000次。
代表性论文：
1.Zheng W, Wang Y, Cui J, Guo G, Hou J,Stewart AF, Zhang Y, Bian X, Wang X (2024)ReaL-MGE; enhanced multiplex genome engineering and application to malonyl-CoA anabolism. Nat Comm. 15:9750.
2.Luan J, Song C, Liu Y, He R, Guo R, Cui Q, Jiang C, Li X, Hao K,Stewart AF, Fu J, Zhang Y, Wang H.(2024)Seamless site-directed mutagenesis in complex cloned DNA sequences using the RedEx method. Nat Protoc. doi: 10.1038/s41596-024-01016-9.
3.Wang X, Zheng W, Zhou H,Tu Q, Tang Y-J, Wu X,Stewart AF, Zhang Y, Bian X.(2022) Improved dsDNA recombineering enables versatile multiplex genome engineering of kilobase-scale sequences in diverse bacteria. Nucleic Acids Res. 50, e15.
4.GoveasN, WaskowC, ArndtK, HeubergerJ, ZhangQ, AlexopoulouD, DahlA, BirchmeierW, AnastassiadisK,StewartAF, KranzA. MLL1 is required for maintenance of intestinal stem cells. (2021) PLoS Genetics, 17, e1009250.
5.Ashokkumar D, ZhangQ,MuchC,BledauAS,Naumann R, Alexopoulou D, Dahl A, Goveas N, FuJ,AnastassiadisK,StewartAFandKranz A (2020)MLL4 is required after implantation whereas MLL3 becomes essential during late gestation. Development 147:dev186999.
6.Schmidt K, Zhang Q, Tasdogan A, Petzold A, Dahl A, Arneth BM, Slany R, Fehling H-J, Kranz A,Stewart AFand Anastassiadis K (2018) The histone 3 lysine 4 methyltransferase Setd1b is essential for hematopoietic stem and progenitor cell homeostasis in mice. eLife, 7, e27157.
7.Arndt K, Kranz A, Fohgrub J, Jolly A, Bledau AS, Di Virgilio M, Lesche M, Dahl A, Höfer T,Stewart AF, Waskow C. (2018)SETD1A protects HSCs from activation-induced functional declinein vivo.Blood 131, 1311-1324.
8.Arndt K, Kranz A, Fohgrub J, Jolly A, Bledau AS, Di Virgilio M, Lesche M, Dahl A, Höfer T,Stewart AF, Waskow C. (2018)SETD1A protects HSCs from activation-induced functional decline in vivo.Blood 131, 1311-1324.
9.Hanna CW, Taudt A, Huang J, Gahurova L, Kranz A, Andrews S, Dean W,Stewart AF, Colomé-Tatché M, Kelsey G. (2018)MLL2 conveys transcription-independent H3K4 trimethylation in oocytes.Nat Struct Mol Biol. 25:73-82
10.Wang H, Li Z, Jia R, Yin J, Li A,Xia L, Yin Y, Müller R, Fu J,Stewart AFand Zhang Y (2018) ExoCET:Exonucleasein vitroassemblycombined with RecET recombination
11.Brici D, Zhang Q, Reinhardt S, Dahl A, Hartmann H, Schmidt K, Goveas N, Huang J, Gahurova L, Kelsey G, Anastassiadis K,Stewart AFand Kranz A. (2017) The histone 3 lysine 4 methyltransferase Setd1b is a maternal effect gene required for the oogenic gene expression program. Development, 144:2606-2617
12.Zhang W, Fu J, Liu J, Wang H, Schiwon M, Janz S, Schaffarczyk L, von der Goltz L, Ehrke-Schulz E, Dörner J, Solanki M, Boehme P, Bergmann T, Lieber A, Lauber C, Dahl A, Petzold A, Zhang Y,Stewart AFand Ehrhardt A. (2017) An engineered adenovirus library as a resource for the spectrum-wide exploration of virus and vector diversity.Cell Rep. 2017 19, 1698-1709.
13.Baker O, Tsurkan S, Fu J, Klink B, Rump A, Obst M, Kranz A, Schröck E, Anastassiadis K andStewart AF(2017) The contribution of homology arms to nuclease-assisted genome engineering. Nucleic Acids Res, 45:8105-8115.
14.Subramaniam S, Erler A, Fu J, Tang J, Gopalswamy M, Ramakrishnan S, Keller A, Grundmeier G, Müller D, Sattler M andStewart AF(2016) DNA annealing by Redbis insufficient for homologous recombination and the additional requirements involve intra- and inter-molecular interactions. Sci Reports 6:34525.
15.Ander M, Subramaniam S, Fahmy K,Stewart AF, Schaeffer E (2015) A single strand annealing protein clamps DNA to detect and secure homology. PLoS Biol. 13(8):e1002213.
16.Bledau A, Schmidt K, Neumann K, Hill U, Ciotta G, Gupta A, Torres DC, Fu J, Kranz A,Stewart AFand Anastassiadis K. (2014) The H3K4 methyltransferase Setd1a is first required at the epiblast stage, whereas Setd1b becomes essential after gastrulation. Development, 141, 1022-35.
17.Denissov S, Hofemeister H, Marks H, Kranz A, Ciotta G, Singh S, Anastassiadis K, Stunnenberg HG andStewart AF. (2014) Mll2 is required for H3K4 trimethylation of bivalent promoters in ES cells whereas Mll1 is redundant. Development, 141, 526-37.
18.WangH, Bian X, Xia L, Ding X, Müller R, Zhang Y, Fu J,Stewart AF.(2014)Improved seamless mutagenesis by recombineering usingccdBfor counterselection.Nucleic Acids Res. 42:e37.
19.Rostovskaya M, Fu J, Obst M, Baer I, Weidlich S, Wang H, Smith AJH, Anastassiadis K andStewart AF. Transposon mediated BAC transgenesis in human ES cells (2012) Nucleic Acids Res. 40, e150.
20.Fu J,Bian X, Hu S, Wang H, Huang F, Seibert PM, Plaza A, Xia L,Müller R,Stewart AFand Zhang Y (2012)Full length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting. Nature Biotechnology, 30, 440-6.
其他学术称号和荣誉
1. 欧洲科学院院士 （The Academia Europaea, MAE）
2. 欧洲分子生物学组织会员（EMBO Fellow）
3. 德累斯顿工业大学生物技术中心终身教授
4. 2010年国际转基因技术协会国际奖（每年仅1人）
发明专利
1. Stewart AF and Logie C. (1993) Regulation of site-specific recombination by site-specific recombinase/nuclear receptor fusion proteins (SSR-LBDs).US Patent 6 04 430.
2. Stewart AF, Zhang Y and Buchholz, F. (1997) Novel DNA cloning method (recombineering). WO/1999/029837.
3. Stewart AF, Zhang Y and Muyrers JJP (1999) Methods and compositions for directed cloning and subcloning using homologous recombination. WO/2001/004288.
4. Stewart AF (1999) Genetic switch. WO/2001/029231.
5. Stewart AF and Buchholz F (2000) Substrate linked directed evolution (SLiDE). WO/2002/044409
6. Muyrers JPP, Zhang Y and Stewart AF (2001) Phage annealing proteins mediate efficient oligonucleotide directed mutagenesis. WO/2002/062988.
7. Stewart AF, Zhang Y, Hallet B (2001) Tyrosine recombinase for genetic engineering US7083976B2.
8. Mueller R, Wenzel SC, Gross F, Stewart AF and Zhang Y (2004) Methods for heterologous expression of secondary metabolites. WO/2006/046152.
9. Hauber I, Hauber J, Stewart AF, Sarkar I, Buchholz F (2007) Use of a tailored recombinase for the treatment of retroviral infections.WO/2008/083931
10. Maresca, M, Erler, AS; Fu, J, Seibert, PM, Stewart, AF, Zhang Y, (2009) Method of nucleic acid recombination. WO/2009/104094.
11.Maresca, M, Noll S, Kranz H, Zhang Y Stewart AF (2009) Methods for site directed mutagenesis. WO/2010/113031.
12. Stewart AF, Iacone, R, Maresca, M, Anastassiadis K. (2009) Method of altering nucleic acids (intron recombineering). WO/2010/140066.
13. Zhang Y, Fu J, Stewart AF (2010) Direct DNA cloning.WO/2011/154927
14. Tu, Q; Zhang, Y; Fu, J; Müller, R; Stewart, AF.(2013) Electrocompetent cells and preparation thereof. WO/2014/060427.
15. Wang H, Zhang Y, Fu J, Stewart AF (2017) ExoCET method for direct DNA cloning - application numbers201710177676.1;XII 2029/17.
招贤纳士
本实验室长期招聘硕士研究生、博士研究生，博士后、科研助理等科研人员。如果你对基因组工程、干细胞调控、表观遗传学等前沿领域充满好奇，渴望探索生命的奥秘，欢迎加入我们的团队，一起开启科学探索之旅。
联系方式：francis.stewart@sdu.edu.cn（请同时转发202585900002@sdu.edu.cn）
