医药微生物技术

霍刘杰

教授
博士/硕士生导师
0532-58632596
个人简介 科研项目 学术论文 其他介绍

教育背景

德国萨尔大学/亥姆霍兹药物研究所,药物生物技术,博士学位

德国萨尔大学/亥姆霍兹药物研究所,药物生物技术,硕士学位

天津大学,生物工程,学士学位


研究工作经历

2017.10-至今,“齐鲁青年学者”特聘教授,微生物技术国家重点实验室,生命科学学院,山东大学

2014.09-2017.09,博士后,美国伊利诺伊大学香槟分校化学系


研究方向

1.微生物天然产物的生物合成

2.合成生物学改造优化微生物天然产物合成

3.基因组挖掘发现新型微生物天然产物

主持课题

2022.01-2024.12,国家自然科学基金委国际(地区)合作与交流项目课题,32161133013,在研,主持

2020.01-2024.12,国家重点研发计划子课题负责人,2019YFA0905702,在研,主持

2019.07-2024.06,国家重点研发计划子课题负责人,2018YFA0900400,在研,主持

2019.07-2022.06,山东省自然科学基金青年项目,ZR2019QC001,在研,主持

2018.07-2021.06,江苏省自然科学基金青年基金项目,BK20180228,在研,主持

2017.10-2022.10,山东大学齐鲁青年学者启动经费,主持

代表性论文

2022:

1.Wang, X.; Song, C.; Qi, Q.; Zhang, Y.*; Li, R.*;Huo, L.*, Biochemical characterization of a polyethylene terephthalate hydrolase and design of high-throughput screening for its directed evolution.Engineering Microbiology2022, 2 (100020), DOI:doi.org/10.1016/j.engmic.2022.100020

2.Han, Y.; Wang, X.; Zhang, Y.;Huo, L.*, Discovery and Characterization of Marinsedin, a New Class II Lanthipeptide Derived from Marine Bacterium Marinicella sediminis F2T.ACS Chemical Biology2022, 17(4):785-790. DOI: 10.1021/acschembio.2c00021.

3.Shen, Q.; Zhou, H.; Dai, G.; Zhong, G.;Huo, L.; Li, A.; Liu, Y.; Yang, M.; Ravichandran, V.; Zheng, Z., Tang J.; Jiao N.; Zhang Y.; Bian X.*, Characterization of a Cryptic NRPS Gene Cluster in Bacillus velezensis FZB42 Reveals a Discrete Oxidase Involved in Methimazole Biosynthesis.ACS Catalysis2022, 3371-3381. DOI: 10.1021/acscatal.1c05131

4.Pang L.; Niu W.; Duan Y.;Huo L.; Li A.; Wu J.; Zhang Y.; Bian X.*; Zhong G.*, In vitro characterization of a nitro-forming oxygenase involved in 3-(trans-2’-aminocyclopropyl) alanine biosynthesis.Engineering Microbiology2022, 2 (100007). DOI: doi.org/10.1016/j.engmic.2021.100007


2020:

1.Wang, Z. J.; Zhou, H.; Zhong, G.;Huo, L.; Tang, Y. J.; Zhang, Y.*; Bian, X.*, Genome Mining and Biosynthesis of Primary Amine-Acylated Desferrioxamines in a Marine Gliding Bacterium.Organic Letters2020, 22 (3), 939-943.DOI: 10.1021/acs.orglett.9b04490

2.Huo, L.; Zhao, X.; Acedo, J. Z.; Estrada, P.; Nair, S. K.; van der Donk, W. A.*, Characterization of a Dehydratase and Methyltransferase in the Biosynthesis of Ribosomally Synthesized and Post-translationally Modified Peptides in Lachnospiraceae.ChemBioChem2020, 21 (1-2), 190-199. DOI: 10.1002/cbic.201900483


2019:

1.Huo, L.; Hug, J. J.; Fu, C.; Bian, X.*; Zhang, Y.*; Müller, R.*, Heterologous expression of bacterial natural product biosynthetic pathways.Natural Product Reports2019, 36 (10), 1412-1436.DOI: 10.1039/c8np00091c

2.Chen, H.; Bian, Z.; Ravichandran, V.; Li, R.; Sun, Y.;Huo, L.; Fu, J.; Bian, X.; Xia, L.; Tu, Q., et al., Biosynthesis of polyketides by trans-AT polyketide synthases in Burkholderiales.Critical Reviews in Microbiology2019, 45 (2), 162-181. DOI: 10.1080/1040841X.2018.1514365

3.Bobeica, S. C.#; Dong, S. H.#;Huo, L.; Mazo, N.; McLaughlin, M. I.; Jimenez-Oses, G.; Nair, S. K.*; van der Donk, W. A.*, Insights into AMS/PCAT transporters from biochemical and structural characterization of a double Glycine motif protease.eLife2019, 8. DOI: 10.7554/eLife.42305


2018:

1.Wang, X.; Zhou, H.; Chen, H.; Jing, X.; Zheng, W.; Li, R.; Sun, T.; Liu, J.; Fu, J.;Huo, L., et al., Discovery of recombinases enables genome mining of cryptic biosynthetic gene clusters in Burkholderiales species.Proceedings of the National Academy of Sciences of the United States of America2018, 115 (18), E4255-E4263. DOI: 10.1073/pnas.1720941115


2017:

1.Huo, L.; Ökesli, A.; Zhao, M.; van der Donk, W. A.*, Insights into the Biosynthesis of Duramycin.Applied and Environmental Microbiology2017, 83 (3).DOI: 10.1128/AEM.02698-16

2.Gemperlein, K.; Hoffmann, M.;Huo, L.; Pilak, P.; Petzke, L.; Müller, R.; Wenzel, S. C.*, Synthetic biology approaches to establish a heterologous production system for coronatines.Metabolic Engineering2017, 44, 213-222.DOI: 10.1016/j.ymben.2017.09.009


2016:

1.Mukherjee, S.;Huo, L.; Thibodeaux, G. N.; van der Donk, W. A.*, Synthesis and Bioactivity of Diastereomers of the Virulence Lanthipeptide Cytolysin.Organic Letters2016, 18 (23), 6188-6191.DOI: 10.1021/acs.orglett.6b03246

2.Mann, G.#;Huo, L.#; Adam, S.; Nardone, B.; Vendome, J.; Westwood, N. J.; Müller, R.; Koehnke, J.*, Structure and Substrate Recognition of the Bottromycin Maturation Enzyme BotP.ChemBioChem2016, 17 (23), 2286-2292.DOI: 10.1002/cbic.201600406

3.Huo, L.; van der Donk, W. A.*, Discovery and Characterization of Bicereucin, an Unusual D-Amino Acid-Containing Mixed Two-Component Lantibiotic.Journal of the American Chemical Society2016, 138 (16), 5254-7. DOI: 10.1021/jacs.6b02513


2012:

1.Huo, L.; Rachid, S.; Stadler, M.; Wenzel, S. C.*; Müller, R.*, Synthetic biotechnology to study and engineer ribosomal bottromycin biosynthesis.Chemistry & Biology(Cell Chemical Biology)2012, 19 (10), 1278-87.DOI: 10.1016/j.chembiol.2012.08.013

2.Barbie, P.;Huo, L.; Müller, R.; Kazmaier, U., Stereoselective synthesis of deuterium-labeled (2S)-cyclohexenyl alanines, biosynthetic intermediates of cinnabaramide.Organic Letters2012, 14 (23), 6064-7. DOI: 10.1021/ol3029548


2011:

1.Rachid, S.;Huo, L.; Herrmann, J.; Stadler, M.; Köpcke, B.; Bitzer, J.; Müller, R.*, Mining the cinnabaramide biosynthetic pathway to generate novel proteasome inhibitors.ChemBioChem2011, 12 (6), 922-31.DOI: 10.1002/cbic.201100024

2.Quade, N.#;Huo, L.#; Rachid, S.; Heinz, D. W.*; Müller, R.*, Unusual carbon fixation gives rise to diverse polyketide extender units. Nature Chemical Biology 2011, 8 (1), 117-24.DOI: 10.1038/nchembio.734


获奖

1.2012年优秀国家自费留学生奖学金,国家留学基金委

2.2012德国微生物协会(VAAM)年会最佳大会报告, Lecture Award.

3.2013欧洲微生物学会会议奖学金,Federation of European Microbiological Societies (FEMS) Meeting Attendance Grant.