医药微生物技术

王海龙

教授
博士/硕士生导师
个人简介 科研项目 学术论文 其他介绍

教育背景

博士硕博连读:2006-2012毕业院校:湖南师范大学专业:微生物学

博士联合培养:2009-2010 德国德累斯顿工业大学 专业:分子生物学

学士起止时间:2002-2006 毕业院校:湖南师范大学 专业:生物基地班

工作经历

起止时间 单位名称 职务

2022-至今,山东大学微生物技术研究院党委委员、党支部书记

2021-至今,山东大学微生物技术国家重点实验室副主任

2021-至今,山东大学微生物技术研究院副院长

2018-至今, 山东大学 微生物技术国家重点实验室 教授、博导

2013-2017,山东大学微生物技术国家重点实验室 博士后

研究方向

1.微生物合成生物学

2.基因编辑技术

3.抗生素药物生物合成

4.工程噬菌体

5.病毒载体疫苗

6.肿瘤靶向细菌

科研项目

1.山东省自然科学杰出青年基金,2023.1-2025.12,主持

2.国家自然科学基金优秀青年基金,2022.1-2024.12,主持

3.山东大学杰出中青年学者(第一层次),2022.1-2026.12,主持

4.山东省泰山学者青年专家(期满考核优秀),2019.1-2023.12,主持

5.山东大学齐鲁青年学者,2018.7-2023.6,主持

6.国家重点研发计划子课题(2019YFA0904001),非天然噬菌体的设计合成,2020.1-2024.12,主持。

7.国家重点研发计划子课题(2018YFA0900403),放线菌底盘适配性机理与产物高产机制研究,2019.7-2024.6,主持。

8.山东省自然科学基金重大基础研究项目(ZR2019ZD22),高效表达非洲猪瘟病毒多抗原的伪狂犬载体构建及评价,2019.12-2024.12,主持。

9.国家自然科学基金青年项目(31700073),植物促生土著链霉菌的人工改良以增强其杀线虫活性,2018.01−2020.12,主持,已结题。

10.中国博士后科学基金特别资助项目(2015T80710),改造埃博霉素生物合成途径以提高单一组分的产量和纯度,2015.07-2017.01,主持,已结题。

11.山东省博士后创新项目(201303110),通过诱发细胞内DNA双链断裂来直接克隆基因组DNA,2013.10-2015.06,主持,已结题。

代表性论文

1.Luan, J.#; Song, C.; Liu, Y.; He, R.; Guo, R.; Cui, Q.; Jiang, C.; Li, X.; Hao, K.; Stewart, A. F.; Fu, J.; Zhang, Y. &Wang, H.*(独立通讯作者),Seamless site-directed mutagenesis in complex cloned DNA sequences using the RedEx method,Nature Protocols,2024,已在线发表.(IF=17.4,生化研究方法一区)

2.Yu, G.#; Duan, Q.; Cui, T.; Jiang, C.; Li, X.; Li, Y.; Fu, J.; Zhang, Y.;Wang, H. *(并列通讯作者)& Luan, J.*, Development of a bacterial gene transcription activating strategy based on transcriptional activator positive feedback,Journal of Advanced Research, 2023, Dec 18:S2090-1232(23)00400-9.(IF=11.0,综合性期刊一区)

3.Li, X.#; Guo, R.; Luan, J.; Fu, J.; Zhang, Y. &Wang, H.*(独立通讯作者), Improving spinosad production by tuning expressions of the forosamine methyltransferase and the forosaminyl transferase to reduce undesired less active byproducts in the heterologous hostStreptomyces albusJ1074,Microbial Cell Factories, 2023, 22(1), 15.(IF=5.5,生物工程与应用微生物一区)

4.Jiang, C.#; She, Q. &Wang, H.*(独立通讯作者), Editorial: Insights in genome editing tools and mechanisms: 2022.Frontiers in Genome Editing, 2023, 5, 1240576.(IF=4.6)

5.李雨桐#,崔天琦,张海林,于广乐,栾霁*,王海龙*(主通讯作者),肿瘤靶向细菌Escherichia coliNissle 1917在癌症治疗中的研究进展,中国生物工程杂志,2023, 43(6): 54-68.

6.姜婵娟#,崔天琦,孙洪娈,焦念志,符军,张友明*,王海龙*(主通讯作者),ExoCET-BAC策略高效抓取和组装高AT含量基因组大片段,合成生物学,2022, 3(1): 238-251.获得“《合成生物学》期刊2024年度优秀论文”

7.Jiang, T.#,*; Yang, X.#; Li, G.; Zhao, X.; Sun, T.; Muller, R.;Wang, H. *(并列通讯作者); Li, M.* & Zhang, Y.*, Bacteria-Based Live Vehicle for In Vivo Bioluminescence Imaging,Analytical Chemistry, 2021, 93(47), 15687-15695.(IF=6.5,化学一区)

8.Tang, Y.#; Zhang, C.; Cui, T.; Lei, P.; Guo, Z. *;Wang, H. *(并列通讯作者)& Liu, Q. *, The Discovery of Actinospene, a New Polyene Macrolide with Broad Activity against Plant Fungal Pathogens and Pathogenic Yeasts,Molecules, 2021, 26(22).(IF=4.6,化学二区)

9.Song, C.#; Luan, J.#; Li, R.; Jiang, C.; Hou, Y.; Cui, Q.; Cui, T.; Tan, L.; Ma, Z.; Tang, Y. J.; Stewart, A. F.; Fu, J.; Zhang, Y. &Wang, H.*(独立通讯作者), RedEx: a method for seamless DNA insertion and deletion in large multimodular polyketide synthase gene clusters,Nucleic Acids Research, 2020, 48(22), e130.(IF=16.1,生化与分子生物学一区)

10.Jiang, C.#; Zhou, H.; Sun, H.; He, R.; Song, C.; Cui, T.; Luan, J.; Fu, J.; Zhang, Y.; Jiao, N.;Wang, H.*(独立通讯作者),Establishing an efficient salinomycin biosynthetic pathway in three heterologousStreptomyceshosts by constructing a 106-kb multioperon artificial gene cluster,Biotechnology & Bioengineering, 2021, 118: 4668-4677. (IF=4.0,生物工程与应用微生物二区)

11.Abbasi, M. N.#; Fu, J.; Bian, X.;Wang, H.*(并列通讯作者); Zhang, Y.* & Li, A.*, Recombineering for Genetic Engineering of Natural Product Biosynthetic Pathways,Trends in Biotechnology, 2020, 38(7), 715-728.(IF=16.8,生物工程与应用微生物一区)

12.Song, C.#; Luan, J.#; Cui, Q.; Duan, Q.; Li, Z.; Gao, Y.; Li, R.; Li, A.; Shen, Y.; Li, Y.; Stewart, A. F.; Zhang, Y.*; Fu, J.* &Wang, H.*(主通讯作者), Enhanced heterologous spinosad production from a 79-kb synthetic multi-operon assembly,ACS Synthetic Biology, 2019, 8(1), 137-147.(IF=4.2,生化研究方法二区)

13.Wang, H.#(第一作者); Li, Z.; Jia, R.; Yin, J.; Li, A.; Xia, L.; Yin, Y.; Muller, R.; Fu, J.*; Stewart, A. F.* & Zhang, Y.*, ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes,Nucleic Acids Research, 2018, 46(5), e28.(IF=16.1,生化与分子生物学一区)

14.Wang, H.#(并列第一作者); Li, Z.#; Jia, R.; Hou, Y.; Yin, J.; Bian, X.; Li, A.; Muller, R.; Stewart, A. F.*; Fu, J.* & Zhang, Y.*, RecET direct cloning and Redab recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression,Nature Protocols, 2016, 11(7), 1175-1190.(IF=17.4,生化研究方法一区)

15.Wang, H.#(第一作者); Bian, X.; Xia, L.; Ding, X.; Müller, R.; Zhang, Y.*; Fu, J.* & Stewart, A. F.*, Improved seamless mutagenesis by recombineering usingccdBfor counterselection,Nucleic Acids Research, 2014, 42(5), e37.(IF=16.1,生化与分子生物学一区)

16.Jing, X.#; Cui, Q.#; Li, X.; Yin, J.; Ravichandran, V.; Pan, D.; Fu, J.; Tu, Q.;Wang, H.*(并列通讯作者); Bian, X.* & Zhang, Y.*, EngineeringPseudomonas protegensPf-5 to improve its antifungal activity and nitrogen fixation,Microbial Biotechnology, 2020, 13(1), 118-133.(IF=5.8,生物工程与应用微生物二区)

17.Luan, J.#; Li, Z.#;Wang, H.*(独立通讯作者); Fu, J. & Zhang, Y.,In vitroassay revealed mismatches between guide RNA and target DNA can enhance Cas9 nuclease activity,Current Chinese Science, 2020, 1(1), 69-72.

18.Zhang, Y.#; Liu, R.#; Tian, K.#; Wang, Z.#; Yang, X.; Gao, D.; Zhang, Y.; Fu, J.*;Wang, H.*(并列通讯作者)& Zhao, J.*, Fiber2 and hexon genes are closely associated with the virulence of the emerging and highly pathogenic fowl adenovirus 4,Emerging Microbes & Infections, 2018, 7(1), 199.(IF=8.3,微生物学二区)

19.Wang, H.#(第一作者); Yao, T.; Cai, M.; Xiao, X.; Ding, X. & Xia, L.*, A genome walking strategy for the identification of nucleotide sequences adjacent to known regions,Biotechnology Letters, 2013, 35(2), 279-284.

20.Yu, G.#; Li, X.; Duan, Q.; Fu, J.; Zhang, Y.;Wang, H.& Luan J.*, Systematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacteriumPseudomonas stutzeriDSM4166 to improve its nitrogenase activity.Microbial Cell Factories, 2023, 22(1): 91.

21.Rostovskaya, M.#, Fu, J., Obst, M., Baer, I., Weidlich, S.,Wang, H., Smith, A. J. H., Anastassiadis, K.* & Stewart, A. F.*, Transposon-mediated BAC transgenesis in human ES cells,Nucleic Acids Research, 2012, 40(19), e150.

22.Zhang, W.#, Fu, J.#, Liu, J.,Wang, H., Schiwon, M., Janz, S., Schaffarczyk, L., von der Goltz, L., Ehrke-Schulz, E., Domer, J., Solanki, M., Boehme, P., Bergmann, T., Lieber, A., Lauber, C., Dahl, A., Petzold, A., Zhang, Y.*, Stewart, A. F.* & Ehrhardt, A.*, An engineered virus library as a resource for the spectrum-wide exploration of virus and vector diversity,Cell Reports, 2017, 19(8), 1698-1709.

23.Zhang, W.#, Fu, J., Liu, J.,Wang, H., Schulz, E., Solanki, M., Boehme, P., Gebbing, M., Bergmann, T., Doerner, J., Stewart, A. F. & Ehrhardt, A.*, High-throughput cloning and tagging of an engineered adenovirus-library empowers vector diversity for broad applications,Molecular Therapy, 2015, 23, S21.

24.Bian, X.#, Tang, B.#, Yu, Y., Tu, Q., Gross, F.,Wang, H., Li, A., Fu, J., Shen, Y., Li, Y., Stewart, A. F., Zhao, G., Ding, X.*, Muller, R.* & Zhang, Y.*, Heterologous production and yield improvement of epothilones inBurkholderialesstrain DSM 7029,ACS Chemical Biology, 2017, 12(7), 1805-1812.

25.Yin, J.#,Wang, H., Li, R., Ravichandran, V., Bian, X., Li, A., Tu, Q., Stewart, A.F., Fu, J.* & Zhang, Y.*, A practical guide to recombineering inPhotorhabdusandXenorhabdus,Current Topics in Microbiology and Immunology, 2017.

26.Ravichandran, V.#, Zhong, L.,Wang, H., Yu, G., Zhang, Y.* & Li, A.*, Virtual screening and biomolecular interactions of CviR-based quorum sensing inhibitors against chromobacterium violaceum,Frontiers in Cellular and Infection Microbiology, 2018, 8, 292.

27.Liu, Q.#, Shen, Q.#, Bian, X., Chen, H., Fu, J.,Wang, H., Lei, P., Guo, Z., Chen, W.*, Li, D.* & Zhang, Y.*, Simple and rapid direct cloning and heterologous expression of natural product biosynthetic gene cluster inBacillus subtilisvia Red/ET recombineering,Scientific Reports, 2016, 6, 34623.

28.Jiang, B.#, Zhang, R., Feng, D., Wang, F., Liu, K., Jiang, Y., Niu, K., Yuan, Q., Wang, M.,Wang, H., Zhang, Y. & Fang, X.*, A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers inPenicillium oxalicum,Frontiers in Microbiology, 2016, 7, 485.

29.Zhu, L.#, Yue, X., Han, K., Li, Z., Zheng, L., Yi, X.,Wang, H., Zhang, Y. & Li, Y.*, Allopatric integrations selectively change host transcriptomes, leading to varied expression efficiencies of exotic genes inMyxococcus xanthus,Microbial Cell Factories, 2015, 14, 105.

30.Bian, X.#, Huang, F.,Wang, H., Klefisch, T., Muller, R.* & Zhang, Y.*, Heterologous production of glidobactins/luminmycins inEscherichia coliNissle containing the glidobactin biosynthetic gene cluster fromBurkholderiaDSM7029,Chembiochem, 2014, 15(15), 2221-2224.

31.Yu, F.#, Jing, X., Li, X.,Wang, H., Chen, H., Zhong, L., Yin, J., Pan, D., Yin, Y., Fu, J., Xia, L., Bian, X., Tu, Q. & Zhang, Y., Recombineering Pseudomonas protegens CHA0: An innovative approach that improves nitrogen fixation with impressive bactericidal potency,Microbiology Research, 2019, 218, 58-65.

32.Fu, J.#; Bian, X.#; Hu, S.;Wang, H.; Huang, F.; Seibert, P. M.; Plaza, A.; Xia, L.; Müller, R.*; Stewart, A. F.* & Zhang, Y.*, Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting,Nature Biotechnology, 2012, 30(5), 440-446.

人才称号

1.山东省杰出青年基金,2022. 9

2.国家自然科学基金委优青,2021.8

3.山东大学杰出中青年学者(第一层次),2022.1

4.山东省泰山学者(青年专家),期满考核优秀,2019.1

5.山东大学齐鲁青年学者,2018.7


获奖情况

1.山东省青年科技奖,2022.5

2.教育部自然科学二等奖,第二位,2019.12

3.《合成生物学》期刊2024年度优秀论文,2024.8

4.山东大学优秀博士学位论文指导奖,2022.12

5.山东大学重大科研突出成绩奖励,2017.12

6.山东省大学生生物学大赛二等奖优秀指导教师,2020.10

7.山东大学青岛校区第三届十佳书院导师,2020.11

8.山东大学青岛校区第二届十佳书院导师,2019.10

9.山东大学微生物技术研究院优秀共产党员,2022.7

10.山东大学微生物技术研究院“科研之星”,2021.1


发明专利

1.New technique for genomic large fragment direct cloning and DNA multi-molecular assembly,欧洲发明专利,授权号:EP3604524B1,张友明;王海龙;符军;A.Francis Stewart

2.Method of homologous recombination of DNA,美国发明专利,申请号:16578385,张友明;王海龙;符军;A.Francis Stewart

3.王海龙,宋超逸,栾霁,符军,张友明.一种大型基因簇的无痕定点改造方法及其应用,中国发明专利,授权号: ZL202010147911.2

4.王海龙,宋超逸,符军,张友明.一种含有多杀菌素多操纵子人工基因簇质粒的构建方法与应用,中国发明专利,授权号: ZL201811407515.8

5.于广乐,王海龙,涂强,张友明.一种施氏假单胞突变菌株及其构建方法和应用,中国发明专利,授权号: ZL202010068324.4

6.基因组大片段直接克隆和DNA多分子组装新技术,中国发明专利,授权号:ZL201710177676.1,张友明;王海龙;符军;A.Francis Stewart