教育背景：

博士 1981.07－1985.06 澳大利亚新南威尔士大学 生物化学

本科 1978.03－1981.02 澳大利亚新南威尔士大学 生物化学

工作经历：

2014.01-2022.03 德国 德累斯顿工业大学（Dresden University of Technology） 生物技术中心主任（Director of BIOTEC）

2005.01-2022.03 德国 德累斯顿工业大学（Dresden University of Technology） W3教授（W3 Professor）

2001.07-2004.12 德国 德累斯顿马普细胞生物学和遗传学研究所（Max-Planck Institute for Cell Biology and Genetics, Dresden） C4教授（C4 Professor）

1991.01-2001.06 德国 海德堡欧洲分子生物学实验室（European Molecular Biology Laboratories, Heidelberg） 组长（Group Leader）

1987.01-1990.12 德国 德国癌症研究中心 (Deutsches Krebsforschungszentrum) 访问学者（Visiting Scholar）

1985.07-1986.12德国德国癌症研究中心(Deutsches Krebsforschungszentrum) 洪堡研究员（Alexander von Humboldt Fellow）

研究方向：

表观遗传学，基因组工程，干细胞系统生物学

近五年论文（2020-2024）：

1.Luan J, Song C, Liu Y, He R, Guo R, Cui Q, Jiang C, Li X, Hao K,Stewart A F, Fu J, Zhang Y, Wang H. Seamless Site-Directed Mutagenesis in Complex Cloned DNA Sequences Using the RedEx Method.Nat. Protoc.2024,19(11), 3360–3388.

2.Al-Fatlawi A, Schroeder M,Stewart A F. The Rad52 SSAP Superfamily and New Insight into Homologous Recombination.Commun. Biol.2023,6, 87.

3.Medina-Ruiz L, Bartolini R, Wilson G J, Dyer D P, Vidler F, Hughes C E, Schuette F, Love S, Pingen M, Hayes A J, Fu J,Stewart A F, Graham G J. Analysis of Combinatorial Chemokine Receptor Expression Dynamics Using Multi-Receptor Reporter Mice.eLife2022,11, e72418.

4.Grinat J, Kosel F, Goveas N, Kranz A, Alexopoulou D, Rajewsky K, Sigal M,Stewart A F, Heuberger J. Epigenetic Modifier Balances Mapk and Wnt Signalling in Differentiation of Goblet and Paneth Cells.Life Sci. Alliance2022,5(4).

5.Hanna C W, Huang J, Belton C, Reinhardt S, Dahl A, Andrews S,Stewart A F, Kranz A, Kelsey G. Loss of Histone Methyltransferase SETD1B in Oogenesis Results in the Redistribution of Genomic Histone 3 Lysine 4 Trimethylation.Nucleic Acids Res.2022,50(4), 1993–2004.

6.Wang X, Zheng W, Zhou H, Tu Q, Tang Y-J,Stewart A F, Zhang Y, Bian X. Improved dsDNA Recombineering Enables Versatile Multiplex Genome Engineering of Kilobase-Scale Sequences in Diverse Bacteria.Nucleic Acids Res.2022,50(3), e15.

7.Michurina A, Sakib M S, Kerimoglu C, Krüger D M, Kaurani L, Islam M R, Joshi P D, Schröder S, Centeno T P, Zhou J, Pradhan R, Cha J, Xu X, Eichele G, Zeisberg E M, Kranz A,Stewart A F, Fischer A. Postnatal Expression of the Lysine Methyltransferase SETD1B Is Essential for Learning and the Regulation of Neuron-Enriched Genes.EMBO J.2022,41(1), e106459.

8.Goveas N, Waskow C, Arndt K, Heuberger J, Zhang Q, Alexopoulou D, Dahl A, Birchmeier W, Anastassiadis K,Stewart A F, Kranz A. MLL1 Is Required for Maintenance of Intestinal Stem Cells.PLOS Genet.2021,17(12), e1009250.

9.Lackner A, Sehlke R, Garmhausen M, Giuseppe Stirparo G, Huth M, Titz‐Teixeira F, van der Lelij P, Ramesmayer J, Thomas H F, Ralser M, Santini L, Galimberti E, Sarov M,Stewart A F, Smith A, Beyer A, Leeb M. Cooperative Genetic Networks Drive Embryonic Stem Cell Transition from Naïve to Formative Pluripotency.EMBO J.2021,40(8), e105776.

10.Grinat J, Heuberger J, Vidal R O, Goveas N, Kosel F, Berenguer-Llergo A, Kranz A, Wulf-Goldenberg A, Behrens D, Melcher B, Sauer S, Vieth M, Batlle E,Stewart A F, Birchmeier W. The Epigenetic Regulator Mll1 Is Required for Wnt-Driven Intestinal Tumorigenesis and Cancer Stemness.Nat. Commun.2020,11(1), 6422.

11.Song C, Luan J, Li R, Jiang C, Hou Y, Cui Q, Cui T, Tan L, Ma Z, Tang Y-J,Stewart A F, Fu J, Zhang Y, Wang H. RedEx: A Method for Seamless DNA Insertion and Deletion in Large Multimodular Polyketide Synthase Gene Clusters.Nucleic Acids Res.2020,48(22), e130.

12.Ramakrishnan S, Subramaniam S, Kielar C, Grundmeier G,Stewart A F, Keller A. Protein-Assisted Room-Temperature Assembly of Rigid, Immobile Holliday Junctions and Hierarchical DNA Nanostructures.Molecules2020,25(21), 5099.

13.Ashokkumar D, Zhang Q, Much C, Bledau A S, Naumann R, Alexopoulou D, Dahl A, Goveas N, Fu J, Anastassiadis K,Stewart A F, Kranz A. MLL4 Is Required after Implantation, Whereas MLL3 Becomes Essential during Late Gestation.Development2020,147(12), dev186999.

14.Cooperative genetic networks drive embryonic stem cell transition from naïve to formative pluripotency. https://doi.org/10.15252/embj.2020105776.