教育背景：

博士 1981.07－1985.06 澳大利亚新南威尔士大学 生物化学

本科 1978.03－1981.02 澳大利亚新南威尔士大学 生物化学

工作经历：

2014.01-2022.03 德国 德累斯顿工业大学（Dresden University of Technology） 生物技术中心主任（Director of BIOTEC）

2005.01-2022.03 德国 德累斯顿工业大学（Dresden University of Technology） W3教授（W3 Professor）

2001.07-2004.12 德国 德累斯顿马普细胞生物学和遗传学研究所（Max-Planck Institute for Cell Biology and Genetics, Dresden） C4教授（C4 Professor）

1991.01-2001.06 德国 海德堡欧洲分子生物学实验室（European Molecular Biology Laboratories, Heidelberg） 组长（Group Leader）

1987.01-1990.12 德国 德国癌症研究中心 (Deutsches Krebsforschungszentrum) 访问学者（Visiting Scholar）

1985.07-1986.12德国德国癌症研究中心(Deutsches Krebsforschungszentrum) 洪堡研究员（Alexander von Humboldt Fellow）

研究方向：

表观遗传学，基因组工程，干细胞系统生物学

参与主持欧洲各国多项科学基金会研究项目，包括六个欧盟综合项目（每个项目涉及超过10 个欧洲合作伙伴和超过 1000 万欧元）、德国DFG科学基金项目等。

主要项目：

（1）海外引才计划创新长期项目,2024-2029,主持

（2）2024-2027德国DFG科学基金项目，STE903/13-1,Trithorax-Group(H3K4甲基转移酶亚类)的核结构研究。30万欧元，主持，在研。

（3）2023-2026德国DFG科学基金项目，STE 903/12-2，H3K4甲基化转移酶MLL3和MLL4对哺乳动物胚胎发育的调节作用。30万欧元，主持，在研。

（4）欧洲干细胞系统生物学项目（SyBoSS），主持，结题

共发表学术论文超过180篇，H指数：98，总引用逾35000次。

近五年论文（2020-2024）：

1.Luan J, Song C, Liu Y, He R, Guo R, Cui Q, Jiang C, Li X, Hao K,Stewart A F, Fu J, Zhang Y, Wang H. Seamless Site-Directed Mutagenesis in Complex Cloned DNA Sequences Using the RedEx Method.Nat. Protoc.2024,19(11), 3360–3388.

2.Al-Fatlawi A, Schroeder M,Stewart A F. The Rad52 SSAP Superfamily and New Insight into Homologous Recombination.Commun. Biol.2023,6, 87.

3.Medina-Ruiz L, Bartolini R, Wilson G J, Dyer D P, Vidler F, Hughes C E, Schuette F, Love S, Pingen M, Hayes A J, Fu J,Stewart A F, Graham G J. Analysis of Combinatorial Chemokine Receptor Expression Dynamics Using Multi-Receptor Reporter Mice.eLife2022,11, e72418.

4.Grinat J, Kosel F, Goveas N, Kranz A, Alexopoulou D, Rajewsky K, Sigal M,Stewart A F, Heuberger J. Epigenetic Modifier Balances Mapk and Wnt Signalling in Differentiation of Goblet and Paneth Cells.Life Sci. Alliance2022,5(4).

5.Hanna C W, Huang J, Belton C, Reinhardt S, Dahl A, Andrews S,Stewart A F, Kranz A, Kelsey G. Loss of Histone Methyltransferase SETD1B in Oogenesis Results in the Redistribution of Genomic Histone 3 Lysine 4 Trimethylation.Nucleic Acids Res.2022,50(4), 1993–2004.

6.Wang X, Zheng W, Zhou H, Tu Q, Tang Y-J,Stewart A F, Zhang Y, Bian X. Improved dsDNA Recombineering Enables Versatile Multiplex Genome Engineering of Kilobase-Scale Sequences in Diverse Bacteria.Nucleic Acids Res.2022,50(3), e15.

7.Michurina A, Sakib M S, Kerimoglu C, Krüger D M, Kaurani L, Islam M R, Joshi P D, Schröder S, Centeno T P, Zhou J, Pradhan R, Cha J, Xu X, Eichele G, Zeisberg E M, Kranz A,Stewart A F, Fischer A. Postnatal Expression of the Lysine Methyltransferase SETD1B Is Essential for Learning and the Regulation of Neuron-Enriched Genes.EMBO J.2022,41(1), e106459.

8.Goveas N, Waskow C, Arndt K, Heuberger J, Zhang Q, Alexopoulou D, Dahl A, Birchmeier W, Anastassiadis K,Stewart A F, Kranz A. MLL1 Is Required for Maintenance of Intestinal Stem Cells.PLOS Genet.2021,17(12), e1009250.

9.Lackner A, Sehlke R, Garmhausen M, Giuseppe Stirparo G, Huth M, Titz‐Teixeira F, van der Lelij P, Ramesmayer J, Thomas H F, Ralser M, Santini L, Galimberti E, Sarov M,Stewart A F, Smith A, Beyer A, Leeb M. Cooperative Genetic Networks Drive Embryonic Stem Cell Transition from Naïve to Formative Pluripotency.EMBO J.2021,40(8), e105776.

10.Grinat J, Heuberger J, Vidal R O, Goveas N, Kosel F, Berenguer-Llergo A, Kranz A, Wulf-Goldenberg A, Behrens D, Melcher B, Sauer S, Vieth M, Batlle E,Stewart A F, Birchmeier W. The Epigenetic Regulator Mll1 Is Required for Wnt-Driven Intestinal Tumorigenesis and Cancer Stemness.Nat. Commun.2020,11(1), 6422.

11.Song C, Luan J, Li R, Jiang C, Hou Y, Cui Q, Cui T, Tan L, Ma Z, Tang Y-J,Stewart A F, Fu J, Zhang Y, Wang H. RedEx: A Method for Seamless DNA Insertion and Deletion in Large Multimodular Polyketide Synthase Gene Clusters.Nucleic Acids Res.2020,48(22), e130.

12.Ramakrishnan S, Subramaniam S, Kielar C, Grundmeier G,Stewart A F, Keller A. Protein-Assisted Room-Temperature Assembly of Rigid, Immobile Holliday Junctions and Hierarchical DNA Nanostructures.Molecules2020,25(21), 5099.

13.Ashokkumar D, Zhang Q, Much C, Bledau A S, Naumann R, Alexopoulou D, Dahl A, Goveas N, Fu J, Anastassiadis K,Stewart A F, Kranz A. MLL4 Is Required after Implantation, Whereas MLL3 Becomes Essential during Late Gestation.Development2020,147(12), dev186999.

14.Cooperative genetic networks drive embryonic stem cell transition from naïve to formative pluripotency. https://doi.org/10.15252/embj.2020105776.

其他学术称号和荣誉

1. 欧洲科学院院士 （The Academia Europaea, MAE）

2. 欧洲分子生物学组织会员（EMBO Fellow）

3. 德累斯顿工业大学生物技术中心终身教授

4. 2010年国际转基因技术协会国际奖（每年仅1人）

发明专利

1. Stewart AF and Logie C. (1993) Regulation of site-specific recombination by site-specific recombinase/nuclear receptor fusion proteins (SSR-LBDs).US Patent 6 04 430.

2. Stewart AF, Zhang Y and Buchholz, F. (1997) Novel DNA cloning method (recombineering). WO/1999/029837.

3. Stewart AF, Zhang Y and Muyrers JJP (1999) Methods and compositions for directed cloning and subcloning using homologous recombination. WO/2001/004288.

4. Stewart AF (1999) Genetic switch. WO/2001/029231.

5. Stewart AF and Buchholz F (2000) Substrate linked directed evolution (SLiDE). WO/2002/044409

6. Muyrers JPP, Zhang Y and Stewart AF (2001) Phage annealing proteins mediate efficient oligonucleotide directed mutagenesis. WO/2002/062988.

7. Stewart AF, Zhang Y, Hallet B (2001) Tyrosine recombinase for genetic engineering US7083976B2.

8. Mueller R, Wenzel SC, Gross F, Stewart AF and Zhang Y (2004) Methods for heterologous expression of secondary metabolites. WO/2006/046152.

9. Hauber I, Hauber J, Stewart AF, Sarkar I, Buchholz F (2007) Use of a tailored recombinase for the treatment of retroviral infections.WO/2008/083931

10. Maresca, M, Erler, AS; Fu, J, Seibert, PM, Stewart, AF, Zhang Y, (2009) Method of nucleic acid recombination. WO/2009/104094.

11.Maresca, M, Noll S, Kranz H, Zhang Y Stewart AF (2009) Methods for site directed mutagenesis. WO/2010/113031.

12. Stewart AF, Iacone, R, Maresca, M, Anastassiadis K. (2009) Method of altering nucleic acids (intron recombineering). WO/2010/140066.

13. Zhang Y, Fu J, Stewart AF (2010) Direct DNA cloning.WO/2011/154927

14. Tu, Q; Zhang, Y; Fu, J; Müller, R; Stewart, AF.(2013) Electrocompetent cells and preparation thereof. WO/2014/060427.

15. Wang H, Zhang Y, Fu J, Stewart AF (2017) ExoCET method for direct DNA cloning - application numbers201710177676.1;XII 2029/17.

招贤纳士

本实验室长期招聘硕士研究生、博士研究生，博士后、科研助理等科研人员。如果你对基因组工程、干细胞调控、表观遗传学等前沿领域充满好奇，渴望探索生命的奥秘，欢迎加入我们的团队，一起开启科学探索之旅。

联系方式：francis.stewart@sdu.edu.cn（请同时转发202585900002@sdu.edu.cn）