教育背景

2006/09 - 2011/06 山东大学，微生物学，博士

2009/12 - 2011/02 德国马普生物物理研究所，分子生物学，联合培养博士

2002/09 - 2006/06 山东师范大学，生物科学，学士

工作经历

起止时间 单位名称 专业技术职务

2024.3至今 山东大学微生物技术研究院 专聘研究员

2017.9至今 山东大学微生物技术研究院 副教授

2012.2-2017.8 山东大学生命科学学院 讲师

2012.2-2014.2 山东大学化学与化工学院 博士后

研究方向

1、蛋白质合成分泌调控机理与应用技术

2、优质酶蛋白挖掘、创制与高效分泌生产菌种构建

3、生物质资源的微生物利用机制及应用

科研项目

1、国家自然科学基金面上项目《里氏木霉非经典分泌途径的机制解析》2026-2029主持，在研

2、山东省重点研发计划乡村振兴科技创新提振行动计划子课题2024-2027 主持，在研

3、山东省自然科学基金面上项目《里氏木霉氮代谢调控因子TAM1调控纤维素酶基因转录表达的机制研究》2024-2027主持，在研

4、国家重点研发计划子课题《丝状真菌蛋白质高效合成分泌机制研究》主持，结题

5、国家自然科学基金面上项目《转录辅抑制复合物Cyc8-Tup1在瑞氏木霉纤维素酶基因转录激活中的作用机制》主持，结题

7、国家自然科学基金青年项目《瑞氏木霉纤维二糖转运蛋白CBT1的结构功能及其在纤维素酶基因诱导表达中的作用机制》主持，结题

8、山东省优秀中青年科学家奖励基金《瑞氏木霉纤维二糖转运系统在纤维素酶诱导表达中的功能研究》主持，结题

代表性论文

1)Wang FC, Li YQ, Wang YL, Xu XD, Cao XN, Cao TS, Zhang WX*, Liu WF. Engineering lipase TLL to improve its acid tolerance and its biosynthesis in Trichoderma reesei for biodiesel production from acidified oil. Bioresource Technology. 2024 Dec:413:131521.

2)Zhang W¹, Li L, Li T, Li X, Wang X, Yao Q, Lu X, Chen G*, Liu W*. SGBP-B-like bimodular cellulose-binding protein CHU_1279 is essential for cellulose utilization by Cytophaga hutchinsonii. Appl Environ Microbiol. 2025 Apr 23;91(4):e0247124.

3)Xu WQ, Ren YJ, Xia YX, Liu L, Meng XF, Chen GJ, Zhang WX*, Liu WF. A novel transcriptional repressor specifically regulates xylanase gene 1 in Trichoderma reesei. Biotechnol Biofuels Bioprod. 2023 Oct 27;16(1):161.

4)Yang RF, Wang ZX, Xia YX, Zheng FL, Kang FS, Meng XF, Zhang WX*, Liu WF. Role of the Nitrogen Metabolism Regulator TAM1 in Regulation of Cellulase Gene Expression in Trichoderma reesei. Appl Environ Microbiol. 2023 Jan 31;89(1):e0142122.

5)Wu XX, Li FZ, Yang RF, Meng XF, Zhang WX*, Liu WF. Identification of a bidirectional promoter from Trichoderma reesei and its application in dual gene expression. Journal of Fungi. 2022 Oct 10;8(10):1059.

6)Zhang WX¹, Guo JQ, Wu XX, Ren YJ, Li CY, Meng XF, Liu WF*. Reformulating the hydrolytic enzyme cocktail of Trichoderma reesei by combining XYR1 overexpression and elimination of four major cellulases to improve saccharification of corn fiber. J Agric Food Chem. 2022 Jan 12;70(1):211-222.

7)Xu WQ, Fang Y, Ding MY, Ren YJ, Meng XF, Chen GJ, Zhang WX*, Liu WF. Elimination of the sugar transporter GAT1 increased xylanase I production in Trichoderma reesei. Frontiers in Microbiology. 2022 Jan 26;13:810066.

8)Wang L¹, Zhang WX¹, Cao YL, Zheng FL, Zhao GL, Lv XX, Meng XF, Liu WF*. Interdependent recruitment of CYC8/TUP1 and the transcriptional activator XYR1 at target promoters is required for induced cellulase gene expression in Trichoderma reesei. PLOS Genetics. 2021 Feb 19;17(2):e1009351.

9)Zhang WX¹, Guo JQ, Wang Z, Li YW, Meng XF, Shen Y, Liu WF*. Improved production of germacrene A, a direct precursor of ß-elemene, in engineered Saccharomyces cerevisiae by expressing a cyanobacterial germacrene A synthase. Microb Cell Fact.2021 Jan 7;20(1):7.

10)An JL, Zhang WX*, Wu WP, Chen GJ, Liu WF. Characterization of a highly stable α-galactosidase from thermophilic Rasamsonia emersonii heterologously expressed in a modified Pichia pastoris expression system. Microbial Cell factories. 2019 Oct 23;18(1):180.

11)Wang L, Lv XX, Cao YL, Zheng FL, Meng XF, Shen Y, Chen GJ, Liu WF*, Zhang WX*. A novel transcriptional regulator RXE1 modulates the essential transactivator XYR1 and cellulase gene expression in Trichoderma reesei. Applied Microbiology and Biotechnology. 2019 Jun;103(11):4511-4523.

12)Wang L, Yang RF, Cao YL, Zheng FL, Meng XF, Zhang WX*, Liu WF*. CLP1, a novel plant Homeo domain protein, participates in regulating cellulase gene expression in the filamentous fungus Trichoderma reesei. Frontiers in Microbiology. 2019 Aug 6;10:1700.

13)Zhou YR, Song XY, Li Y, Shi JC, Shi WL, Chen XL, Liu WF, Liu XM, Zhang WX*, Zhang YZ*. Enhancing peptaibols production in the biocontrol fungus Trichoderma longibrachiatum SMF2 by elimination of a putative glucose sensor. Biotechnology and Bioengineering. 2019 Nov;116(11):3030-3040.

14)Wang X¹, Zhang WX¹, Zhou H, Chen GJ*, Liu WF*. An extracytoplasmic function sigma factor controls cellulose utilization by regulating the expression of an outer membrane protein in Cytophaga hutchinsonii. Appl Environ Microbiol 2019;85(5).

15)Cao YL, Zheng FL, Wang L, Zhao GL, Chen GJ, Zhang WX*, Liu WF*. Rce1, a novel transcriptional repressor, regulates cellulase gene expression by antagonizing the transactivator Xyr1 in Trichoderma reesei. Molecular Microbiology. 2017 Jul;105(1):65-83