微生物技术研究院学术委员会

李越中

教授
主任
0532-58631539
个人简介 学术论文 其他介绍

教育背景

博士 起止时间:1990-1993,毕业院校:山东大学,专业:微生物学

硕士 起止时间:1987-1990,毕业院校:山东农业大学,专业:真菌学

学士 起止时间:1983-1987,毕业院校:山东大学,专业:微生物学


工作经历

起止时间 单位名称 专业技术职务

1993-1995 中科院沈阳应用生态所 博士后

1995-2000 山东大学微生物技术国家重点实验室 副教授

1996.4-1996.10 德国国家生物技术研究中心(GBF) 高级访问学者

2000-今 山东大学微生物技术国家重点实验室 教授


研究方向

黏细菌(Myxobacteria)在系统进化上属于Proteobacteria类群的δ分支。在其生活史中表现复杂的多细胞群体行为特性,显示与多细胞真核生物较多的相似性,显著不同于其他细菌,被认为是生活于多细胞生命边缘的单细胞生物类群,是一类“高等”的原核生物,是细菌细胞间通讯与协作,多细胞形态发生,以及生命进化等研究的重要模式生物材料。黏细菌能够降解利用其他活的微生物细胞、细胞残体以及许多难降解的大分子有机物,是一类捕食性细菌,在微生物生态系统扮演重要角色。黏细菌拥有庞大的基因组,是目前报道的原核生物中最大的。黏细菌能够产生丰富的次级代谢产物,具有种类多、结构新、作用机理新颖多样的特性,被公认是继放线菌之后又一个重要的药源微生物新类群。

我们以黏细菌为基本材料,开展如下方面的研究:

1.黏细菌资源及其多样性,包括可培养和未培养的黏细菌类群;

2.以黏细菌资源为基础,开展黏细菌细胞行为及其进化研究;

3.以黏细菌产生的抗肿瘤化合物埃博霉素(Epothilones)为主要研究对象,开展黏细菌发酵工程和代谢工程研究;

4.在黏细菌中建立和改进遗传操作体系,并对黏细菌进行遗传分析和遗传改造。

代表性论文(近五年)

Li Zhuo, Tian-Yu Wan, Zhuo Pan, Jia-Ning Wang, Duo-Hong Sheng, Yue-Zhong Li*. A Dual-Functional Orphan Response Regulator Negatively Controls the Differential Transcription of Duplicate groELs and Plays a Global Regulatory Role in Myxococcus.mSystems. 2022 Apr 26; 7(2): e0105621.

Shuge Wu, Jingjing Wang*, Jianing Wang, Xinran Du, Qi Ran, Qi Chen, Duohong Sheng, Yue-Zhong Li*. Halalkalibacterium roseum gen. nov., sp. nov., a new member of the family Balneolaceae isolated from soil.International Journal of Systematic and Evolutionary Microbiology. 2022 Apr; 72(4).

Changsheng Wu*,#, Yinzhen Yin#, Le-Le Zhu, Youming Zhang, Yue-Zhong Li*. Metagenomic Sequencing Driven Multidisciplinary Approaches to Shed Light on The Untapped Microbial Natural Products.Drug Discovery Today2022 Mar; 27(3): 730-742.

Li-Juan Zhang, De-Gao Wang, Peng Zhang, Changsheng Wu*, Yue-Zhong Li*. Promiscuity Characteristics of Versatile Plant Glycosyltransferases for Natural Product Glycodiversification.ACS Synthetic Biology. 2022 Feb 18; 11(2): 812-819.

Peng Zhang, Lijuan Zhang, Xukai Jiang, Xiao-Tong Diao, Shuang Li, Dan-Dan Li, Zheng Zhang, Junqiang Fang, Ya-Jie Tang, Da-Lei Wu, Changsheng Wu*, Yue-Zhong Li*. Docking-guided rational engineering of a macrolide glycosyltransferase glycodiversifies epothilone B.Communications Biology. 2022 Jan 27; 5(1): 100.

Xin-ran Du, Qi Ran, Jia-ning Wang, Hong Jiang, Jing-jing Wang*, Yue-Zhong Li*. Microvirga roseola sp. nov. and Microvirga lenta sp. nov., isolated from Taklamakan Desert soil.International Journal of Systematic and Evolutionary Microbiology. 2022 Jun; 72(6).

Zheng Zhang#,*, Ya Liu#, Peng Zhang, Jianing Wang, Dandan Li, Yue-zhong Li*. PAAR proteins are versatile clips that enrich the antimicrobial weapon arsenals of prokaryotes.mSystems2021 Dec 21; 6(6): e0095321.

Dan-dan Li, Jin-lan Wang, Ya Liu, Yue-zhong Li*, Zheng Zhang*. Expanded analyses of the functional correlations within structural classifications of glycoside hydrolases.Computational and Structural Biotechnology Journal2021 Nov 2; 19: 5931-5942.

Wei-Feng Hu#, Luo Niu#, Xin-jing Yue, Le-Le Zhu, Wei Hu, Yue-Zhong Li*, Changsheng Wu*. Characterization of Constitutive Promoters for The Elicitation of Secondary Metabolites in Myxobacteria.ACS Synthetic Biology2021, 10, 2904−2909.

De-Gao Wang#, Luo Niu#, Zhao-Min Lin, Jing-Jing Wang, Dong-Fang Gao, Hai-Yan Sui, Yue-Zhong Li*, Changsheng Wu*. The Discovery and Biosynthesis of Nicotinic Myxochelins from an Archangium sp. SDU34.Journal of Natural Products2021, 84, 2744−2748.

Ye Wang, Xin-jing Yue*, Shu-fei Yuan, Yu Hong, Wei-feng Hu, Yue-zhong Li*. Internal promoters and their effects on the transcription of operon genes for the epothilone production in Myxococcus xanthus.Frontiers in Bioengineering and Biotechnology2021 Oct 27;9. doi: 10.3389/fbioe.2021.758561

Jingjing Wang, Qi ran, Xinran Du, Shuge Wu, Jianing Wang, Duohong Sheng, Qi Chen, Zongjun Du, Yue-zhong Li*. Two new Polyangium species, P. aurulentum sp. nov. and P. jinanense sp. nov., isolated from a soil sample.Systematic and Applied Microbiology2021, 44, 126274.

Duohong Sheng#, Xiaojing Chen#, Yajie Li, Jingjing Wang, Li Zhuo, Yue-zhong Li*. ParC, a new partitioning protein, is necessary for the active form of ParA from Myxococcus pMF1 plasmid.Frontiers in Microbiology2021, 11:623699.

Yuelan Li#, Li Zhuo#, Xiaobin Li, Yongqiang Zhu, Shuge Wu, Wei Hu, Tao Shen, Yue-Zhong Li*, changsheng Wu*. Myxadazoles, myxobacterium-derived isoxazole-benzimidazole hybrids with cardiovascular activities.Angewandte Chemie International Edition2021, 60, 21679 –21684.

Jingjing Wang#, Jianing Wang#, Shuge Wu, Zheng Zhang*, Yue-zhong Li*. Global geographic diversity and distribution of the myxobacteria.Microbiology Spectrum2021, 9: e00012-21.

Jianing Wang, Lidong Wang, Weifeng Hu, Zhuo Pan, Peng Zhang, Chuandong Wang, Jingjing Wang, Shuge Wu, Yue-zhong Li*. Assembly processes and source tracking of planktonic and benthic bacterial communities in the Yellow River estuary.Environmental Microbiology2021, 23(5), 2578–2591.

Jia-Qi Hu#, Jing-Jing Wang#, Yue-Lan Li, Li Zhuo, Ai Zhang, Hai-Yan Sui, Xiao-Ju Li, Tao Shen, Yizhen Yin, Zhi-Hong Wu, Wei Hu, Yue-Zhong Li*, Changsheng Wu*. Combine NMR-basedmetabolic profiling andgenome mining for the accelerated discovery of archangiumide, an allenic macrolide from the myxobacteriumArchangium violaceum SDU8.Organic Letters2021, 23, 2114−2119.

Ya Liu, Jianing Wang, Zheng Zhang, Feng Wang, Ya Gong, Duo-hong Sheng, Yue-zhong Li*. Two PAAR proteins with different C-terminal extended domains have distinct ecological functions in Myxococcus xanthus.Applied and Environmental Microbiology2021, 87: e00080-21.

Duohong Sheng*, Ye Wang, Zhiwei Jiang, Dongkai Liu, Yuezhong Li*. ImuA facilitates SOS mutagenesis by inhibiting RecA-mediated activity in Myxococcus xanthus.Applied and Environmental Microbiology2021, doi:10.1128/AEM.00919-21.

Zhuo Pan, Zheng Zhang, Li Zhuo, Tian-yu Wan, Yue-zhong Li*. Bioinformatic and functional characterization of Hsp70s in Myxococcus xanthus.mSphere2021, 6: e00305-21.

Duo-hong Sheng#*, Ye Wang#, Shu-ge Wu, Rui-xin Duan, Yue-zhong Li*. The regulation of LexA on UV induced SOS response in Myxococcus xanthus based on transcriptome analysis.Journal of Microbiology and Biotechnology, 2021, 31(6).

Zheng Zhang#*, Jianing Wang#, Jinlan Wang, Jingjing Wang, Yuezhong Li*. Estimate of the sequenced proportion of the global prokaryotic genome.Microbiome. 2020 Sep 16;8(1):134.

Wang, J. N., Wang, J. J., Zhang, Z., Li, Z., Li, Y. Z*. Shifts in the Bacterial Population and Ecosystem Functions in Response to Vegetation in the Yellow River Delta Wetlands.mSystems, 2020, 5(3): e00412-20

Y Liu, Z Zhang, F Wang, DD Li, YZ Li*. Identification of type VI secretion system toxic effectors using adaptors as markers.Computational and Structural Biotechnology Journal, 2020, 18(6):3723-3733.

DH Sheng, YX Wang, M Qiu, JY Zhao, YZ Li*. Functional Division Between the RecA1 and RecA2 Proteins in Myxococcus xanthus.Frontiers in Microbiology, 2020, 11:140.

XJ Chen, Z Zhang, YJ Li, L Zhuo, DH Sheng, YZ Li*. Insights into the persistence and phenotypic effects of the endogenous and cryptic plasmid pMF1 in its host strain Myxococcus fulvus 124B02.FEMS Microbiology Ecology, 2020(3):3.

Peng Zhang, Lijuan Zhang, Xin-jing Yue, Ya-jie Tang, Changsheng Wu*, Yue-zhong Li*. Effects of glycosylation on the bioactivity of rapamycin.Applied Microbiology and Biotechnology, 2020, 104(21):9125–9134

SG Wu, JJ Wang, JN Wang, Q Chen, YZ Li*. Psychroflexus aurantiacus sp. nov. isolated from soil in the Yellow River Delta wetlands.International Journal of Systematic and Evolutionary Microbiology, 2020, 70(12).

SG Wu, JJ Wang, JN Wang, Q Chen, YZ Li*. Paucihalobacter ruber gen. nov. sp. nov. isolated from a haloalkaline lake sediment sample.International Journal of Systematic and Evolutionary Microbiology, 2020, 70(10).

JJ Wang, SG Wu, Q Chen, DH Sheng, YZ Li*. Deinococcus terrestris sp. nov. a gamma ray- and ultraviolet-resistant bacterium isolated from soil.International Journal of Systematic and Evolutionary Microbiology, 2020, 70(9).

Peng Zhang, Zheng Zhang, Zhi-feng Li, Qi Chen, Yao-yao Li, Ya Gong, Xin-jing Yue, Duo-hong Sheng, You-ming Zhang, Changsheng Wu*, Yue-zhong Li*. Phylogeny-guided characterization of glycosyltransferases for epothilone glycosylation.Microbial Biotechnology, 2019, 12(4), 763-774

Mian Nabeel Anwar, Zhi Feng Li, Gong Ya, Raghvendra Partap Singh, Yue-zhong Li*. Omics studies revealed the factors involved in the formation of colony boundary in Myxococcus xanthus.Cells, 2019, 8, 530.

Li Zhuo, Zheng Zhang, Zhuo Pan, Duo-hong Sheng, Wei Hu, Yue-zhong Li*. CIRCE element evolved for the coordinated transcriptional regulation of bacterial duplicate groELs.Biochimica et Biophysica Acta - Gene Regulatory Mechanisms. 2018 Oct;1861(10):928-937.

Ya Gong, Zheng Zhang, Ya Liu, Xiu-wen Zhou, Mian Nabeel Anwar, Ze-shuo Li, Wei Hu, Yue-zhong Li*. A nuclease-effector and immunity system for the kin discrimination in Myxococcus xanthus.Environmental Microbiology. 2018, doi: 10.1111/1462-2920.14282.

Ya Gong, Zheng Zhang, Xiu-wen Zhou, Mian Nabeel Anwar, Xiao-zhuang Hu, Ze-shuo Li, Xiao-jing Chen, Yue-zhong Li*. Competitive interactions between incompatible mutants of the social bacterium Myxococcus xanthus DK1622.Frontiers in Microbiology. 2018, 9:1200.

Zheng Zhang, Jin-Lan Wang, Ya Gong, Yue-Zhong Li*. Contributions of substitutions and indels to the structural variations in ancient protein superfamilies.BMC Genomics. 2018 Oct 24;19(1):771.

Li Y, Liu Y, Zhang Z, Chen X, Gong Y and Li Y*. A Post-segregational Killing Mechanism for Maintaining Plasmid PMF1 in Its Myxococcus fulvus Host.Frontiers in Cellular and Infection Microbiology. 2018 Aug 7; 8:274.

JJ Wang, Q Chen, YZ Li*. Chryseolinea flava sp. nov., a new species of Chryseolinea isolated from soil.International Journal of Systematic and Evolutionary Microbiology. 2018 Nov;68(11):3518-3522.

XJ Yue, XW Cui, Z Zhang, WF Hu, ZF Li, YM Zhang and YZ Li*. Effects of transcriptional mode on promoter substitution and tandem engineering for the production of epothilones in Myxococcus xanthus.Applied Microbiology Biotechnology. (2018) doi.org/10.1007/s00253-018-9023-4

R Peng, Y Wang, WW Feng, XJ Yue, JH Chen, XZ Hu, ZF Li, DH Sheng, YM Zhang and YZ Li*. CRISPR/dCas9‑mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus.Microbial Cell Factories(2018) 17:15.

L Zhuo, Y Wang, Z Zhang, J Li, XH Zhang, YZ Li*. Myxococcus xanthus DK1622 coordinates expressions of the duplicate groEL and single groES genes for synergistic functions of GroELs and GroES.Frontiers in Microbiology(2017) 8: 733.

R Peng, JH Chen, WW Feng, Z Zhang, J Yin, ZS Li, YZ Li*. Error-prone DnaE2 balances the genome mutation rates in Myxococcus xanthus DK1622.Frontiers in Microbiology(2017) 8: 122.

XJ Yue, XW Cui, Z Zhang, R Peng, P Zhang, ZF Li, YZ Li*. A bacterial negative transcription regulator binding on an inverted repeat in the promoter for epothilone biosynthesis.Microbial Cell Factories(2017) 16: 92.

YJ Yang, Y Wang, ZF Li, Y Gong, P Zhang, WC Hu, DH Sheng and YZ Li*. Increasing on target cleavage efficiency for CRISPR/Cas9 induced large fragment deletion in Myxococcus Xanthus.Microbial Cell Factories(2017) 16:142.

获奖情况

山东省科技发明二等奖(2006)

国家杰出青年科学基金资助(2008)

“百千万”工程国家级人才(2009)

教育部高等学校自然科学一等奖(2017)

山东省首批领军人才创新工作室(2017)