微生物学、生物化学与分子生物学

李瑞娟

长聘副研究员
硕士生导师
个人简介 科研项目 学术论文 其他介绍

教育背景:

博士:2009.09-2014.07 山东大学 天然药物化学

联合培养博士:2012.10-2014.02 美国北卡罗来纳大学教堂山分校 药物化学

学士:2005.09-2009.07 山东大学 药学


工作经历:

起止时间 单位名称 专业技术职务

2022.09至今 山东大学 微生物技术国家重点实验室 长聘副研究员

2018.03-2022.08 山东大学 微生物技术国家重点实验室 助理研究员

2015.10-2016.01 德国萨尔大学 访问学者

2014.09-2018.02 山东大学 生命科学学院 博士后


研究方向:

微生物基因组编辑系统的建立及其应用

微生物活性天然产物的挖掘及其生物合成

组蛋白去乙酰化酶抑制剂类抗肿瘤药物的高效生物智造


科研项目:

2023.12-2028.11,国家重点研发计划“前沿生物技术”重点专项-子课题,主持。

2021.01-2023.12,山东省自然科学基金面上项目,主持。

2020.01-2024.12,国家重点研发计划“合成生物学”重点专项-子课题,主持。

2018.06-2021.06,山东大学基本科研业务费资助项目,主持。

2016.01-2018.12,国家自然科学基金青年项目,主持。

2015.06-2017.05,中国博士后基金面上资助项目,主持。

2015.01-2016.12,天然产物化学生物学教育部重点实验室开放课题,主持。


代表性论文:

Wentao Zheng#, Yandong Xia#, Xue Wang, Shiqing Gao, Diao Zhou, Vinothkannan Ravichandran, Chanjuan Jiang, Qiang Tu, Yulong Yin, Youming Zhang, Jun Fu*,Ruijuan Li*, Jia Yin*. Precise genome engineering in Pseudomonas using phage-encoded homologous recombination and Cascade-Cas3 system.Nat Protoc2023, 18(9): 2642-2670.

Qingshu Liu#,Ruijuan Li#,*, Hongbo Shi, Runyu Yang, Qiyao Shen, Qingwen Cui, Xiuling Wang, Aiying Li, Youming Zhang*, Jun Fu*. A recombineering system in Bacillus subtilis based on the native phage recombinase pair YqaJ/YqaK.Engineering Microbiology2023, 3(3): 100099.

Ruijuan Li, Aiying Li, Youming Zhang, Jun Fu*. The emerging role of recombineering in microbiology.Engineering Microbiology2023, 3(3): 100097.

Kai Gong, Maoqin Wang, Qiong Duan, Gang Li, Daojing Yong, Cailing Ren, Yue Li, Qijun Zhang, Tao Sun, Huanyun Zhang, Qiang Tu, Changsheng Wu, Jun Fu, Aiying Li, Chaoyi Song*, Youming Zhang*,Ruijuan Li*. High-yield production of FK228 and new derivatives in aBurkholderiachassis.Metab Eng2023, 75(2023): 131‒142.

Zhilong Bian, Shanshan Li, Runyu Yang, Jia Yin, Youming Zhang, Qiang Tu*, Jun Fu*,Ruijuan Li*. Development of a new recombineering system forAgrobacteriumspecies.Appl Environ Microbiol.2022, 88(5): e0249921.

Daojing Yong, Yue Li, Kai Gong, Yingying Yu, Shuai Zhao, Qiong Duan, Cailing Ren, Aiying Li, Jun Fu, Jinfeng Ni*, Youming Zhang*andRuijuan Li*. Biocontrol of strawberry gray mold caused byBotrytis cinereawith the termite associatedStreptomycessp. sdu1201 and actinomycin D.Front Microbiol.2022, 13:1051730.

Khorshed Alam, Yiming Zhao, Kai Gong, Lin Zhong, Md. Mahmudul Islam, Saiful Islam, Geng Li,Ruijuan Li*, Youming Zhang*, Aiying Li*. Isolation, complete genome sequencing and in silico genome mining ofBurkholderiafor secondary metabolites.BMC Microbiol2022, 22:323.

Khorshed Alam#, Jinfang Hao#, Lin Zhong, Guoqing Fan, Qing Ouyang, Md. Mahmudul Islam, Saiful Islam, Hongluan Sun, Youming Zhang,Ruijuan Li*, Aiying Li*. Complete genome sequencing and in silico genome mining reveal the promising metabolic potential inStreptomycesstrain CS-7.Front Microbiol.2022, 13:939919.

Wentao Zheng#, Yandong Xia#, Xue Wang, Shiqing Gao, Diao Zhou, Jun Fu*,Ruijuan Li*, Jia Yin*. Cascade-Cas3 facilitates high-accuracy genome engineering inPseudomonasusing phage-encoded homologous recombination.Engineering Microbiology2022, 2(4): 100046.

Kai Gong#, Daojing Yong#, Jun Fu, Aiying Li, Youming Zhang,Ruijuan Li*. Diterpenoids fromStreptomyces: Structures, Biosyntheses and Bioactivities.ChemBioChem.2022, 23(17): e202200231.

Xiaotong Wang, Chaoyi Song, Qingsheng Qi, Youming Zhang*,Ruijuan Li*, Liujie Huo*. Biochemical characterization of a polyethylene terephthalate hydrolase and design of a high-throughput screening for its directed evolution.Engineering Microbiology2022, 2(2), 100020.

Ruijuan Li#, Hongbo Shi#, Xiaoyu Zhao, Xianqi Liu, Qiong Duan, Chaoyi Song, Hanna Chen, Wentao Zheng, Qiyao Shen, Maoqin Wang, Xue Wang, Kai Gong, Jia Yin, Youming Zhang*, Aiying Li*, Jun Fu*. Development and application of an efficient recombineering system forBurkholderia glumaeandBurkholderia plantarii.Microb Biotechnol2021, 14(4): 1809‒1826.

Khorshed Alam, Md. Mahmudul Islam, Caiyun Li, Sharmin Sultana, Lin Zhong, Qiyao Shen, Guangle Yu, Jinfang Hao, Youming Zhang,Ruijuan Li*, Aiying Li*. Genome mining ofPseudomonasspecies: diversity and evolution of metabolic and biosynthetic potential.Molecules.2021, 26, 7524.

Khorshed Alam, Md. Mahmudul Islam, Kai Gong, Muhammad Nazeer Abbasi,Ruijuan Li*, Youming Zhang*, Aiying Li*. In silico genome mining of potential novel biosynthetic gene clusters for drug discovery fromBurkholderiabacteria.Comput Biol Med.2021, 140 (2022) 105046.

Caiyun Li, Khorshed Alam, Yiming Zhao, Jinfang Hao, Qing Yang, Youming Zhang*,Ruijuan Li*, Aiying Li*. Mining and biosynthesis of bioactive lanthipeptides from microorganisms.Front Bioeng Biotech2021, 9(639): 692466.

Ruijuan Li#, Linda Helbig#, Jun Fu, Xiaoying Bian, Jennifer Herrmann, Michael Baumann, A. Francis Stewart, Rolf Müller, Aiying Li*, Daniel Zips*, and Youming Zhang*. Expressingcytotoxiccompounds inEscherichia coliNissle 1917 fortumor-targetingtherapy.Res Microbiol2019, 170(2): 74–79.

Ruijuan Li#, Song Wang#, Gang Li, Jinchuan Zhou, Jiaozhen Zhang, Youming Zhang, Guosheng Shi, and Hongxiang Lou*.Four new kaurane diterpenoids from the Chinese liverwortJungermannia comataNees.Chem Biodivers2016, 13(12): 1685–1690.

Ruijuan Li, Yu Zhao, Harukuni Tokuda, Xiaoming Yang, Yuehu Wang, Qian Shi, Susan L. Morris-Natschke, Hongxiang Lou*, and Kuo-Hsiung Lee*. Total synthesis of plagiochin G and its derivatives as potential cancer chemopreventive agents.Tetrahedron Lett2014, 55(2014): 6500−6503.

Song Wang,#Ruijuan Li,#Rongxiu Zhu, Jinchuan Zhou, Zhaomin Lin, Jiaozhen Zhang, Jingyi Wu, Yaqi Kang, Susan L. Morris-Natschke, Kuo-Hsiung. Lee, and Hongxiang Lou*. Notolutesins A−J, dolabrane-type diterpenoids from the Chinese liverwortNotoscyphus lutescens.J Nat Prod2014, 77(9): 2081−2087.

Ruijuan Li, Rongxiu Zhu, Yu Zhao, Susan L. Morris-Natschke, Chin-Ho Chen, Song Wang, Jiaozhen Zhang, Jinchuan Zhou, Hongxiang Lou*, and Kuo-Hsiung Lee*. Two new cadinane-type sesquiterpenes from the Chinese liverwortFrullania serrata.Nat Prod Res2014, 28: 1519−1524.

Ruijuan Li, Yi Sun, Bin Sun, Xiaoning Wang, Shanshan Liu, Jinchuan Zhou, Jianping Ye, Yu Zhao, Lei Liu, Kuo-Hsiung Lee, and Hongxiang Lou*. Phytotoxiccis-clerodane diterpenoids from the Chinese liverwortScapania stephanii.Phytochemistry2014, 105(2014): 85−91.

Ruijuan Li,#Zhaomin Lin,#Yaqi Kang, Yanxia Guo, Xin Lv, Jinchuan Zhou, Song Wang, and Hongxiang Lou*. Cembrane-type diterpenoids from the Chinese liverwortsChandonanthus hirtellusandC. birmensis.J Nat Prod2014, 77(2): 339−345.

Ruijuan Li, Rongxiu Zhu, Yaoyao Li, Jinchuan Zhou, Jiaozhen Zhang, Song Wang, Jianping Ye, Yuehu Wang, Susan L. Morris-Natschke, Kuo-Hsiung Lee, and Hong-Xiang Lou*. Secondary metabolites from the Chinese liverwortCephaloziella kiaeri.J Nat Prod2013,76(9): 1700−1708.

Ruijuan Li, Dongxiao Guo, and Hongxiang Lou*. A new guaiane-type sesquiterpene lactone from the Chinese liverwortPorella acutifoliasubsp.tosana.Chin J Nat Med2013,11(1): 0074−0076.

所获专利:

李瑞娟,宫恺,王茂芹,宋超逸,张友明,符军,李爱英。一株高产FK228的工程菌株及其构建与应用。授权号ZL202210109315.4

符军,李瑞娟,边志龙,李珊珊,杨润雨,涂强,张友明。农杆菌同源重组系统及其应用。授权号:ZL202111088227.2

符军,李瑞娟,史洪波,赵晓雨,张友明。伯克氏菌同源重组系统及其应用。授权号ZL202011131275.0

张友明,李瑞娟,高运生,涂强,王宗杰。一株高效异源表达Disorazole Z的工程菌株和构建该菌株的基因簇及其应用。授权号:ZL201711363593.8

张友明,李瑞娟,宋超逸,涂强。一株异源表达组蛋白去乙酰化酶抑制剂的工程菌株及其应用。授权号:ZL201710813845.6