研究方向
1. 非常规酵母代谢工程与合成生物学
2. 大宗化学品的绿色生物制造
科研项目
1. 2023.01-2025.12，解脂耶氏酵母还原性丁二酸合成途径的构建及代谢调控研究，国家自然科学基金青年项目（22208192），主持
2. 2022.10-2025.12，酵母单细胞蛋白与高附加值饲料添加剂联产关键技术及产品，山东省重点研发计划子课题，主持
3. 2021.12-2024.11，塑料生物解聚途径从头设计与解聚微生物重构-2，国家重点研发计划子课题（2021YFC2103604-2），主持
4.2019.11-2021.11，高产丁二酸解脂耶氏酵母细胞工厂的构建研究，青岛市博士后应用研究项目，主持
5. 2019.09-2021.11，重构解脂耶氏酵母中心碳代谢促进丁二酸的高效生物合成，中国博士后科学基金面上项目（2019M662340），主持
代表性论文
1. Liu X,Cui Z, Su T, Lu X, Hou J, Qi Q. Identification of genome integration sites for developing a CRISPR-based gene expression toolkit inYarrowia lipolytica. Microb Biotechnol. 2022 Aug;15(8):2223-2234.
2. Zhang J,Cui Z, Zhu Y, Zhu Z, Qi Q, Wang Q. Recent advances in microbial production of high-value compounds in the tetrapyrrole biosynthesis pathway. Biotechnol Adv. 2022 Mar-Apr;55:107904.
3. Cui Z, Zhu Z, Zhang J, Jiang Z, Liu Y, Wang Q, Hou J, Qi Q. Efficient 5-aminolevulinic acid production through reconstructing the metabolic pathway in SDH-deficientYarrowia lipolytica. Biochem Eng J. 2021;174:108125.
4. Jiang Z#,Cui Z#, Zhu Z, Liu Y, Tang Y, Hou J, Qi Q. Engineering ofYarrowia lipolytica transporters for high-efficient production of biobased succinic acid from glucose. Biotechnol Biofuels. 2021 Jun 27;14(1):145.
5. Cui Z#, Zheng H#, Jiang Z, Wang Z, Hou J, Wang Q, Liang Q, Qi Q. Identification and characterization of the mitochondrial replication origin for stable and episomal expression in Yarrowia lipolytica. ACS Synth Biol. 2021 Apr 16;10(4):826-835.
6. Cui Z, Zheng H, Zhang J, Jiang Z, Zhu Z, Liu X, Qi Q, Hou J. A CRISPR/Cas9-mediated, homology-independent tool developed for targeted genome integration inYarrowia lipolytica. Appl Environ Microbiol. 2021 Feb 26;87(6):e02666-20.
7. Li C, Ong KL,Cui Z, Sang Z, Li X, Patria RD, Qi Q, Fickers P, Yan J, Lin CSK. Promising advancement in fermentative succinic acid production by yeast hosts. J Hazard Mater. 2021 Jan 5;401:123414.
8. 朱子薇,张健,王倩,侯进,崔志勇*,祁庆生*.卟啉代谢途径高价值产物及其微生物合成研究进展.中国科学:生命科学. 2020.
9. Liu Y#, Jiang X#,Cui Z, Qi Q, Hou J. Engineering the oleaginous yeastYarrowia lipolytica for production of α-farnesene. Biotechnol Biofuels. 2019 Dec 23;12:296.
10. Zhao C,Cui Z, Zhao X, Zhang J, Zhang L, Tian Y, Qi Q, Liu J. Enhanced itaconic acid production inYarrowia lipolytica via heterologous expression of a mitochondrial transporter MTT. Appl Microbiol Biotechnol. 2019 Mar;103(5):2181-2192.
11. Cui Z, Jiang Z, Zhang J, Zheng H, Jiang X, Gong K, Liang Q, Wang Q, Qi Q. Stable and efficient biosynthesis of 5-aminolevulinic acid using plasmid-freeEscherichia coli. J Agric Food Chem. 2019 Feb 6;67(5):1478-1483.
12. Cui Z, Jiang X, Zheng H, Qi Q, Hou J. Homology-independent genome integration enables rapid library construction for enzyme expression and pathway optimization inYarrowia lipolytica. Biotechnol Bioeng. 2018; 2, 354-363.
13. Yu Q,Cui Z, Zheng Y, Huo H, Meng L, Xu J, Gao C. Exploring succinic acid production by engineeredYarrowia lipolytica strains using glucose at low pH. Biochem Eng J. 2018;139:51-6.
14. Cui Z, Gao C, Li J, Hou J, Lin C, Qi Q. Engineering of unconventional yeastYarrowia lipolytica for efficient succinic acid production from glycerol at low pH. Metab Eng. 2017; 42, 126-133.
15. Gao C, Yang X, Wang H, Rivero CP, Li C,Cui Z, Qi Q, Lin CSK. Robust succinic acid production from crude glycerol using engineeredYarrowia lipolytica. Biotechnol Biofuels. 2016 Aug 30;9(1):179.
获奖情况
1. 2017.10山东省研究生优秀科技创新成果奖 三等奖
2. 2021.09中国•山东（青岛）博士后创新创业成果大赛产业特别奖
所获专利
1.一种利用具有还原TCA途径的解脂耶氏酵母菌株好氧合成琥珀酸的方法，CN201711276705.6
2.一种利用解脂耶氏酵母途径定位合成萜类化合物的方法，CN201910384517.8
3.具有线粒体定位还原TCA途径的高产琥珀酸酵母工程菌株及其构建方法和应用，202210395975.3
