教育背景

博士1994.9-1997.6 山东大学 微生物学

硕士1991.9-1994.7 山东大学 微生物学

学士1987.9-1994.9 山东大学 微生物工程

工作经历

起止时间 单位名称 专业技术职务

2005.9-今 山东大学微生物技术国家重点实验室 教授

2002.4-2004.5 英国癌症研究基金会伦敦研究所 Research Fellow

1999.9-2005.8 山东大学微生物技术国家重点实验室 副教授

1998.10-2001.2 美国麻省理工学院 博士后

1997.9-1999.8 山东大学微生物技术国家重点实验室 讲师

 研究方向

1.真菌基因表达调控机制，包括丝状真菌纤维素酶基因表达中的信号传导及

转录调控

2.高效真菌底盘及其合成生物学

3.真菌生物活性物质的合成调控

科研项目

1. 国家自然科学基金，瑞氏木霉TrUBC介导的泛素化修饰在纤维素酶基因转录调控中的作用机制

2. 国家自然科学基金，类转运蛋白受体CRT1在瑞氏木霉纤维素酶基因表达中的信号感应传递机制

3. 国家自然科学基金，丝状真菌瑞氏木霉纤维素酶基因转录激活模式分子机制的研究

4. 973计划项目子课题，木质纤维素生物降解多样性、新降解因子及相关机理

5. 新世纪人才资助计划，木霉纤维素酶基因转录表达调控

6. 山东省杰出青年基金，真核微生物遗传学

7. 国际合作项目（英国壳牌石油），Expression and Mechanism Study of Fungi Expansin-like Swollenin

8. 国家自然科学基金，结晶纤维素水解过程中外切葡聚糖酶持续催化机制的研究

9. 国家自然科学基金，泛素-蛋白酶体对转录激活因子Gal4调节机制的研究

10. 863计划，酶法裂解制备酵母胞壁免疫寡糖

代表性论文

1. Cao Y, Zheng F, Wang L, Zhao G, Chen G, Zhang W*,Liu W*. Rce1, a novel transcriptional repressor, regulates cellulase gene expression by antagonizing the transactivator Xyr1 in Trichoderma reesei. Mol Microbiol. 2017, 105(1):65-83

2. Wang L, Zheng F, Zhang W, Zhong Y, Chen G,Meng X* and Liu W.Acopper controlled RNA interference system for characterizing gene functions inTrichoderma reesei.Biotechnology for Biofuels 2018, 11:33

3. Wang Z, An N, Xu W, Zhang W, Meng X, Chen G andLiu W*.Functional characterization of the upstream components of the Hog1-like kinase cascade in hyperosmotic and carbon sensing inTrichoderma reesei.Biotechnology for Biofuels 2018, 11:97

4. Zheng F, Cao Y, Wang L, Lv X, Meng X, Zhang W, Chen G, Liu W*. The mating type locus protein MAT1-2-1 of Trichoderma reesei interacts with Xyr1 and regulates cellulase gene expression in response to light. Scientific Reports 2017, 7(1):17346

5. Zhang F, Cao Y, Lv X, Wang L, Li C, Zhang W, Chen G,liu W*. A copper-responsive promoter replacement system to investigate gene functions in Trichoderma reesei: a case study in characterizing SAGA genes. Appl Microbiol Biotechnol 2016, 101(5):2076-2078

6. Zhou H, Wang X, Yang T, Zhang W, Chen G,Liu W*. An Outer Membrane Protein Involved in the Uptake of Glucose Is Essential for Cytophaga hutchinsonii Cellulose Utilization. Applied and Environmental Microbiology 2016, 82(6):1933-1934.

7. Lv X, Zheng F, Li C, Zhang W, Chen G, Liu W*. Characterization of a copper responsive promoter and its mediated overexpression of the xylanase regulator 1 results in an induction-independent production of cellulases in Trichoderma reesei. Biotechnology for Biofuels 2015, 8(1):67

8. Zhang W, Cao Y, Gong J, Bao X, Chen G,Liu W*. Identification of residues important for substrate uptake in a glucose transporter from the filamentous fungus Trichoderma reesei. Scientific Reports 2015, 5:13829

9. Zhang X, Zhong Y, Yang S, Zhang W, Xu M, Ma A, Zhuang G, Chen G,Liu W*. Diversity and dynamics of the microbial community on decomposing wheat straw during mushroom compost production. Bioresource Technology 2014, 17:183-195

10. Xu J，Zhao G, Kou Y, Zhang W, Zhou Q, Chen G,Liu W*. Intracellular β-glucosidases CEL1a and CEL1b are essential for cellulase induction on lactose in Trichoderma reesei. Eukaryotic Cell 2014, 13(8):1001-1013

11. Qi F, Zhang W, Zhang F, Chen G,Liu W*. Deciphering the effect of the different N-glycosylation sites on the secretion, activity and stability of cellobiohydrolase I from Trichoderma reesei. Apllied and Environmental Microbiology 2014, 80 (13): 3962-3971

12. Zhang W, Kou Y, Xu J, Cao Y, Zhao G, Shao J, Wang H, Wang Z, Bao X, Chen G,Liu W*. Two Major Facilitator Superfamily Sugar Transporters from Trichoderma reesei and Their Roles in Induction of Cellulase Biosynthesis. Journal of Biological Chemistry 2013, 288:32861-32872.

13. Zhou Q, Xu J, Kou Y, Lv X，Zhang X, Zhao G，Zhang W, Chen G,Liu W*. Differential involvement of β-glucosidases from Hypocrea jecorina in rapid induction of cellulase genes by cellulose and cellobiose. Eukaryotic cell 2012, 11: 1371-1381.

14. Li Y, Chen G,Liu WF*. Multiple metabolic signals influence GAL gene activation by modulating the interaction of Gal80p with the transcription activator Gal4p. Molecular Microbiology 2010, 78(2): 414-428.

所获专利

1.葡糖糖酸内酯在诱导纤维素酶表达中的应用，专利号201310705686.X

2. β-葡萄糖苷酶Mut1b及其表达基因与应用，专利号201110451440.5

3. 一种利用微生物酶系对啤酒酵母细胞进行破壁裂解的方法，专利号ZL200810238547.X

4. β-葡萄糖苷酶Cel1b及其表达基因与应用，专利号201110450999.6