医药微生物技术

杜磊

研究员
博士/硕士生导师
0532-58632496
个人简介 科研项目 学术论文 其他介绍

教育背景

博士:2015.09-2018.01,中国科学院大学,微生物学

硕士:2009.09-2012.07,天津大学化工学院,制药工程

学士:2005.09-2009.07,天津大学化工学院,制药工程

 

工作经历

起止时间 单位名称 专业技术职务

2020.07-今,山东大学微生物技术研究院,研究员

2018.01-2020.07,中国科学院,博士后

2013.01-2015.08,中国科学院,研究助理


研究方向

1.微生物合成生物学、天然产物生物合成

2. 生物催化与转化

3.细胞色素P450酶的酶学与酶工程

4.工业微生物菌株改良


科研项目

1.国家重点研发计划“合成生物学”重点专项,2020-2025,骨干

2.山东省自然科学基金重大基础研究项目,2021-2023,主持

3.国家博士后创新人才支持计划(“博新计划”),2018-2020,主持

4.国家自然科学基金青年科学基金,2019-2021,主持

5.中国博士后面上基金,2019-2020,主持

6.山东省自然科学基金博士基金,2019-2022,主持

7.中国科学院优秀博士后基金,2019-2020,主持

8.青岛市博士后应用研究基金,2019-2020,主持

代表性论文

1. Dong S.#, Chen J.#, Zhang X., Guo F., Ma L., You C., Wang X., Zhang W., Wan X., Liu S.-J., Yao L.-S., Li S.,Du L.*, and Feng Y.* Structural basis forselective oxidation of phosphorylated ethylphenolsby cytochrome P450 monooxygenase CreJ.Applied and Environmental Microbiology, 2021, 87: e00018-21. (IF: 4.792)

2. Du L., Li S.* Compartmentalized biosynthesis of fungal natural products.Current Opinion in Biotechnology, 2021, 69: 128-135. (IF: 9.74)

3. Ye Y.#,Du L.#, Zhang X., Newmister S.A.,McCauley M., Alegre-Requena J.V.,Zhang W., Mu S., Minami A.,Fraley A.E.,Adrover-Castellano M.L.,Carney N.A., Shende V.V.,Qi F.,Oikawa H.,Kato H., Tsukamoto S., Paton R.S.,Williams R.M.*, Sherman D.H.*, and Li S.* Fungal-derived brevianamide assembly by a stereoselective semipinacolase.Nature Catalysis, 2020, 3: 497-506.(IF: 41.813)

4. Zhang W.#,Du L.#, Li F., Zhang X., Qu Z., Han L., Li Z., Sun J., Qi F., Yao Q., Sun Y., Geng C., and Li S.* Mechanistic insights into interactions between bacterial class I P450 enzymes and redox partners.ACS Catalysis, 2018, 8: 9992-10003. (IF: 13.084)

5. Du L.#, Dong S.#, Zhang X., Jiang C., Chen J., Yao L., Wang X., Wan X., Liu X., Wang X., Huang S., Cui Q., Feng Y.*, Liu S.-J.*, and Li S.* Selective oxidation of aliphatic C-H bonds in alkylphenols by a chemomimetic biocatalytic system.Proceedings of the National Academy of Sciences of the United States of America, 2017, 114: E5129-E5137.(IF: 11.205)

6. Du L., Ma L., Qi F., Zheng X., Jiang C., Li A., Wan X., Liu S.-J.*, and Li S.* Characterization of a unique pathway for 4-cresol catabolism initiated by phosphorylation inCorynebacterium glutamicum.Journal of Biological Chemistry, 2016, 291: 6583-6594. (IF: 5.157)

7. Du L., Liu R.-H., Ying L., and Zhao G.-R.* An efficient intergeneric conjugation of DNA fromEscherichia coli to mycelia of the lincomycin-producerStreptomyces lincolnensis.International Journal of Molecular Sciences, 2012, 13: 4797-4806. (IF: 5.923)

8. Li, Z., Li, S.,Du, L., Zhang, X., Jiang, Y., Liu, W., Zhang, W., and Li, S. Engineering bafilomycin high-producers by manipulating regulatory and biosynthetic genes in the marine bacteriumStreptomyces lohii.Marine Drugs, 2021, 19: 29. (IF: 5.118)

9. Li S.*,Du L., and Bernhardt R.* Redox Partners: Function modulators of bacterial P450 enzymes.Trends in Microbiology, 2020, 28: 445-454. (IF: 17.079)

10. Fraley A.E., Tran H.T., Kelly S.P., Newmister S.A., Tripathi A., Kato H., Tsukamoto S.,Du L., Li S., Williams R.M.*, and Sherman D.H.* Flavin-dependent monooxygenases NotI and NotI’ mediate spiro-oxindole formation in biosynthesis of the notoamides.ChemBioChem, 2020, 21: 2382-2382. (IF: 3.146)

11. Zhang W.,Du L., Qu Z., Zhang X., Li F., Li Z., Qi F., Wang X., Jiang Y., Men P., Sun J., Cao S., Geng C., Qi F., Wan X., Liu C., and Li S.* Compartmentalized biosynthesis of mycophenolic acid. Proceedings of the National Academy of Sciences of the United States of America, 2019, 116: 13305-13310.(IF: 11.205)

12. Xu H., Liang W., Ning L., Jiang Y., Yang W., Wang C., Qi F., Ma L.,Du L., Fourage L., Zhou Y.J., and Li S.* Directed evolution of P450 fatty acid decarboxylases via high-throughput screening towards improved catalytic activity.ChemCatChem, 2019, 6. (IF: 5.686)

13. Li Z.,Du L., Zhang W., Zhang X., Jiang Y., Liu K., Men P., Xu H., Fortman J. L., Sherman D. H., Yu B., Gao S., and Li S.* Complete elucidation of the late steps of bafilomycin biosynthesis inStreptomyces lohii.Journal of Biological Chemistry, 2017, 292: 7095-7104. (IF: 5.157)

14. Sun Y., Ma L., Han D.,Du L., Qi F., Zhang W., Sun J., Huang S., Kim E.-S., and Li S.* In vitro reconstitution of the cyclosporine specific P450 hydroxylases using heterologous redox partner proteins.Journal of Industrial Microbiology and Biotechnology, 2017, 44: 161-166. (IF: 3.346)

15. Zhang X., Li Z.,Du L., Chlipala G. E., Lopez P. C., Zhang W., Sherman D. H.*, and Li S.* Identification of an unexpected shunt pathway product provides new insights into tirandamycin biosynthesis.Tetrahedron Letters, 2016, 57: 5919-5923. (IF: 2.415)

16. Ma L.,Du L., Chen H., Sun Y., Huang S., Zheng X., Kim E.-S.*, and Li S.* Reconstitution of thein vitro activity of the cyclosporine-specific P450 hydroxylase fromSebekia benihana and development of a heterologous whole-cell biotransformation system.Applied and Environmental Microbiology, 2015, 81: 6268-6275. (IF: 4.792)

17. Pang A.,Du L., Lin C., Qiao J., and Zhao G.-R.* Co-overexpression oflmbW andmetK led to increased lincomycin A production and decreased byproduct lincomycin B content in an industrial strain ofStreptomyces lincolnensis. Journal of Applied Microbiology, 2015, 119: 1064-1074. (IF: 3.772)

18. Yan J.*, Zheng X.,Du L., and Li S.* Integrated lipase production and in situ biodiesel synthesis in a recombinantPichia pastoris yeast: an efficient dual biocatalytic system composed of cell free enzymes and whole cell catalysts.Biotechnology for Biofuels, 2014, 7: 55. (IF: 6.04)

19. 钟贝芬,杜磊,李众,张伟,张兴旺,李菁,黄淑兰,胡翔,李盛英,刘琨。基于细胞色素P450单加氧酶介导的4-甲酚氧化降解途径的天麻素生物合成。湖南师范大学自然科学学报,2018, 41: 33-40.

20. 赵广荣*杜磊,祝琳琪。组合突变提高重组人干扰素α2b的抗病毒活性。生物医学工程学杂志,2011, 28: 347-351.


所获专利

1. 李盛英,李众,杜磊,张兴旺,张伟。一种巴弗洛霉素高产工程菌及其构建和应用。2018, CN201811634377

2. 李盛英,杜磊,马圆圆,徐晓庆,王玉亭。一种多氧霉素I组分高产菌株的筛选方法。2017, CN201710564816

3. 李盛英,杜磊,刘双江。一种酚类化合物氧化的生物催化系统及其应用。2016, CN201610015698

4. 李盛英,房博,齐凤霞,张伟,杜磊。支撑P450脂肪酸脱羧酶活性的还原伴侣蛋白组合及其应用。2016, CN201610151612

5. 赵广荣,张和平,梁玲玲,杜磊,祝琳琪。重组人干扰素α2b DNA片段及编码的蛋白质。2008, CN101434957A

6. 赵广荣,张和平,梁玲玲,杜磊,祝琳琪。一种重组人干扰素α2b DNA片段及编码的蛋白质。2008, CN101906424A