医药微生物技术

卞小莹

教授
博士/硕士生导师
0532-67721928
个人简介 科研项目 学术论文 其他介绍
教育背景

博士2008.10-2013.02德国萨尔大学药物生物技术

硕士2005.09-2008.07 西北农林科技大学 植保资源利用

学士2001.09-2005.07 西北农林科技大学 植物保护


工作经历

起止时间 单位名称 专业技术职务

2015.07-至今 山东大学微生物技术国家重点实验室 教授、博导

2014.10-2015.06 山东大学生命科学学院 副研究员

2013.02-2014.09 德国亥姆霍兹萨尔药物研究所 博士后


研究方向

1.微生物基因组编辑及底盘构建技术

2.微生物天然产物挖掘与改造

3. 微生物天然产物生物合成及合成生物学

科研项目

2023/01-2027/12,山东大学杰出中青年学者,主持

2021/07-2024/06高效微生物细胞工厂的设计原理与构建方法,国家重点研发计划,2021YFC2100500,骨干

2021/01-2024/12伯克氏菌中新型非核糖体脂肽的定向挖掘与途径解析,国家自然科学基金面上项目,32070060,主持

2020/01-2024/12课题1:天然产物“暗物质”定向挖掘的平台技术, 国家重点研发计划课题,2019YFA0905701,主持

2019/07-2022/06微生物次级代谢,山东省自然科学杰出青年基金,ZR2019JQ11,主持

2017/09-2019/08, 兼具生物固氮/杀虫抑菌功能的根际细胞工厂的构建, 山东省自然科学基金重大基础研究项目,ZR2017ZB0212,主持

2017/01-2020/12,一个用于表达黏细菌天然产物的底盘菌的优化及应用,国家自然科学基金面上项目,31670098,主持

2016/01-2018/12,创新霉素生物合成途径的阐释和异源表达,国家自然科学基金青年项目,31500033,主持

2015/7-2020/7,山东大学齐鲁青年学者学科建设经费,主持

代表性论文

Chen H#, Zhong L#, Zhou H#, Sun T, Zhong G, Tu Q, Zhuang Y, Bai X, Wang X, Xu J, Xia L, Shen Y, Zhang Y*,Bian X*.Biosynthesis of Glidomides and Elucidation of Different Mechanisms for Formation of β-OH Amino Acid Building Blocks.Angew Chem Int Ed. 2022, 61(35): e202203591.Highlighted as theFrontispiece.

LiuJ, Wang X,DaiG, Zhang Y,Bian X*.Microbial Chassis Engineering Drives Heterologous Production of Complex Secondary Metabolites.Biotechnol Adv. 2022,59: 107966.

Shen Q#, Zhou H#, Dai G, Zhong G, Huo L, Li A, Liu Y, Yang M, Ravichandran V, Zheng Z, Tang YJ, Jiao N, Zhang Y,Bian X*. Characterization of a Cryptic NRPS Gene Cluster in Bacillus velezensis FZB42 Reveals a Discrete Oxidase Involved in Multithiazole Biosynthesis.ACS Catalysis. 2022, 12(6): 3371-3381.HighlightinHot off the PressofNatural Product Reports.

Wang X#, Zheng W#, Zhou H, Tu Q, Tang YJ, Stewart AF*, Zhang Y*,Bian X*. Improved dsDNA recombineering enables versatile multiplex genome engineering of kilobase-scale sequences in diverse bacteria.Nucleic Acids Res. 2022, 50(3): e15.

Zhang X, Xu X, You C, Yang C, Guo J, Sang M, Geng C, Cheng F, Du L, Shen Y, Wang S, Lan H, Yang F, Li Y, Tang YJ, Zhang Y,Bian X*, Li S*, Zhang W*. Biosynthesis of chuangxinmycin featuring a deubiquitinase-like sulfurtransferase.Angew Chem Int Ed. 2021,60(46): 24418-24423. Recommended inFaculty OpinionsandHot off the PressofNatural Product Reports.

Liu J, Zhou H, Yang Z, Wang X, Chen H, Zhong L, Zheng W, Niu W, Wang S, Ren X, Zhong G, Wang Y, Ding X, Müller R, Zhang Y,Bian X*. Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria.Nat Commun. 2021, 12: 4347.被Nat Commun编辑选为“生物技术与方法”高亮文章.

Zhong L#,DiaoX#,Zhang N#, Li F, Zhou H, Chen H, Bai X, Ren X, Zhang Y*, Wu D*,Bian X*.Engineering and Elucidation of the Lipoinitiation Process in Nonribosomal Peptide Biosynthesis.Nat Commun.2021, 12: 296.被Nat Commun编辑选为“有机化学与化学生物学”高亮文章.

Liu Y#, Zhou H#, Shen Q, Dai G, Yan F, Li X, Ren X, Sun Q, Tang YJ, Zhang Y,Bian X*. Discovery of Polycyclic Macrolide Shuangdaolides by Heterologous Expression of a Cryptic trans-AT PKS Gene Cluster.Org Lett. 2021. 23(17): 6967-6971.

Ouyang Q, Wang X, Zhang N, Zhong L, Liu J, Ding X, Zhang Y,Bian X*.Promoter screening facilitates heterologous production of complex secondary metabolites in Burkholderiales strains.ACS Synth Biol.2020, 9(2):457-460.

Wang ZJ, Zhou H, Zhong G, Huo L, Tang YJ, Zhang Y*,Bian X*. Genome Mining and Biosynthesis of Primary Amine-Acylated Desferrioxamines in a Marine Gliding Bacterium.Org Lett. 2020, 22(3): 939-943.

Zheng W#, Wang X#, Zhou H, Zhang Y*, Li A*,Bian X*. Establishment of recombineering genome editing system in Paraburkholderia megapolitana empowers activation of silent biosynthetic gene clusters.Microb Biotechnol.2020, 13(2): 397-405.获Microb Biotechnol(2020, 13:1702-1704)的Highlight栏目重点介绍。

Jing X#, Cui Q#, Li X, Yin J, Ravichandran V, Pan D, Fu J, Tu Q, Wang H*,Bian X*, Zhang Y*. Engineering Pseudomonas protegens Pf-5 to improve its antifungal activity and nitrogen fixation.Microb Biotechnol. 2020, 13(1): 118-133.

Huo L, Hug JJ, Fu C,Bian X*, Zhang Y*, Müller R*. Heterologous expression of bacterial natural product biosynthetic pathways.Nat Prod Rep.2019, 36(10):1412-1436.入选ESI热点论文和高被引论文

Wang X#, Zhou H#, Chen H#, Jing X, Zheng W, Li R, Sun T, Liu J, Fu J, Huo L, Li YZ, Shen Y, Ding X, Müller R,Bian X*, Zhang Y*. Discovery of recombinases enables genome mining of cryptic biosynthetic gene clusters in Burkholderiales species.Proc Natl Acad Sci U S A. 2018, 115 (18): E4255-E4263.获Curr Opin Microbiol和Curr OpinChemBiol重点推荐。

Bian X#, Tang B#, Yu Y, Tu Q, Gross F, Wang H, Li A, Fu J, Shen Y, Li YZ, Stewart AF, Zhao G, Ding X*, Müller R*, Zhang Y*.Heterologous production and yields improvement of epothilones in the Burkholderiales strain DSM 7029.ACS Chem Biol.2017; 12 (7): 1805-1812. Highlight in Hot off the Press of Natural Product Reports

Bian X, Plaza A, Zhang Y*, Müller R*. Two more pieces of the colibactin genotoxin puzzle from Escherichia coli show incorporation of an unusual 1-aminocyclopropanecarboxylic acid moiety.Chem Sci.2015, 6(5): 3154-3160,

Bian X, Plaza A, Zhang Y*, Müller R*. Luminmycins A-C, cryptic natural products from Photorhabdus luminescens identified by heterologous expression in Escherichia coli.J Nat Prod. 2012, 75(9):1652-1655. 2012 Arthur E. Schwarting Award for Best Paper in the Journal of Natural Products

Fu J#,Bian X#, Hu S, Wang H, Huang F, Seibert PM, Plaza A, Xia L, Müller R*, Stewart AF*, Zhang Y*. Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting.Nat Biotechnol. 2012, 30(5): 440-446. Highlighted inCover Paper’ and ‘News and Views’ of Nature Biotechnology, and Research Highlighted in Nature Methods

获奖情况

2022年山东省和山东大学优秀博士学位论文指导教师

2022年第十三届青岛市青年科技奖

2021年度微生物技术研究院“科研之星”

2022Natural Product ReportsEmerging Investigator Lectureship提名

2021年第五届微生物技术国家重点实验室青年人才学术交流会优秀报告一等奖

2019年度教育部自然科学二等奖,Red/ET基因组编辑与微生物资源发掘,排名第三

2015年中国微生物学会学术年会优秀学术论文交流奖(中国微生物学会)

2013 DECHMA travel grant for 1st European Conference on Natural Products (德国化学工程与生物技术协会)

2012 Arthur E. Schwarting Award for Best Paper in the Journal of Natural Products (美国生药学会)



所获专利

卞小莹,武大雷,钟林,刁晓彤,张娜,张友明。控制脂肽脂链长度改变的关键氨基酸位点及其突变体和应用,ZL202011423361.9

卞小莹,钟林,张娜,陈汉娜,武大雷,张友明。控制脂肽脂链长度改变的关键交换结构域及其突变体和应用,ZL202011422022.9

卞小莹,王雪,陈汉娜,周海波,张友明。一组伯克氏菌同源重组酶及其表达载体与应用,ZL201710685563.2

卞小莹,张友明,许晓坤。创新霉素生物合成基因簇及其应用,ZL201710146841.7

Y. Zhang, J. Fu,X. Bian, F. Stewart, R. Müller.Heterologous hosts.US 9,580,717 B2