教育背景

博士起止时间：1994-1997 毕业院校：山东大学 专业：微生物学

硕士起止时间：1991-1994 毕业院校：山东大学 专业：微生物学

学士起止时间：1987-1991 毕业院校：山东大学 专业：微生物学

工作经历

起止时间 单位名称 专业技术职务

1997－今 山东大学微生物技术国家重点实验室 教授

2006 美国加州大学洛杉矶分校 医学院 访问学者

2002- 2003 英国谢菲尔德大学 生化与分子生物学系 访问学者

研究方向

1. 生物质资源的转化利用及相关酶的分子催化机制

2. 高等真菌生物活性物质的发掘和综合利用

3. 用于控制耐药菌感染的新型噬菌体裂解酶的开发和分子改造

近五年主持的科研项目

1. 2021-2024，工业微生物基因组编辑方法的开发及应用--子课题

国家重点研发计划子课题（主持）

2. 2020-2024，非天然噬菌体的设计合成--人工噬菌体与宿主的互作及临床前应用评价--子课题，国家重点研发计划子课题（主持）

3. 2018-2021, CHU_3237介导的纤维素酶在哈氏噬纤维菌表面锚定机制,国家自然科学基金(主持)

4. 2018-2022，利用非同源末端连接的基因组精简策略构建最适细胞工厂，国家自然基金重点项目（第二位）

5. 2017-2019,新型多孔菌发酵生产天然γ-癸内酯，山东省重点研发计划（主持）

6. 2014-2017，哈氏噬纤维菌新型蛋白分泌系统PorSS的组成以及效应蛋白的鉴定和穿膜识别序列研究，国家自然科学基金(主持)

5. 2012-2015，哈氏噬纤维菌外膜纤维素结合蛋白吸附和解构结晶纤维素机理的研究，国家自然科学基金(主持)

6. 2013-2018，高吸附性能高强度琼脂糖分离介质的开发横向课题(主持)

7. 2011-2015，哈氏噬纤维细菌结晶纤维素降解机制，国家973子课题(主持)

代表性论文

近年作为责任作者的代表性论文：

1. Lijuan Gao, Yahong Tan, Weican Zhang, Qingsheng Qi andXuemei Lu*,Cytophaga hutchinsoniiSprA and SprT are essential components of the type IX secretion system required for Ca2+ acquisition, cellulose degradation, and cell motility,Frontiers in Microbiology, 2021. 12: p. 628555.

2. Dong Zhao, Ying Wang, Sen Wang, Weican Zhang, Qingsheng Qi,Xuemei Lu*.A Disulfide Oxidoreductase CHU_1165 is Essential for cellulose degradation by affecting outer membrane proteins inCytophaga hutchinsonii,Applied and Environmental Microbiology,2020, 86(8): e02789-19

3. Lijuan Gao, Zhiwei Guan, Peng Gao, Weican Zhang, Qingsheng Qi,Xuemei Lu*.Cytophaga hutchinsoniigldN, Encoding a core component of the type IX secretion system, is essential for ion assimilation, cellulose degradation, and cell motility.Applied and Environmental Microbiology. 2020,86(11): e00242-20

4. Xifeng Wang, Dong Zhao, Lijuan Gao, Weican Zhang,Xuemei Lu*. Cage-like polyhedrons of DegQ fromCytophaga hutchinsoniishow stable proteolytic activity and strong chaperone activity,Biochemical Engineering Journal,2020, 159: 107585

5.Sen Wang, Dong Zhao, Weican Zhang,Xuemei Lu*.Identification of a cell-surface protein involved in glucose assimilation and disruption of the crystalline region of cellulose byCytophaga hutchinsonii.Journal of Industrial Microbiology & Biotechnology,2019, 46(11): 1479–1490.

6. Zhiwei Guan, Ying Wang, Lijuan Gao, Weican Zhang andXuemei Lu*. Effects of the histone-like protein HU on cellulose degradation and biofilm formation ofCytophaga hutchinsonii.Applied Microbiology and Biotechnology,2018,102(15): 6593–6611

7. Xifeng Wang, Zhiquan Wang, Xinfeng Bai, Yue Zhao, Weican Zhang andXuemei Lu*. Deletion of a Gene Encoding a Putative Peptidoglycan-Associated Lipoprotein Prevents Degradation of the Crystalline Region of Cellulose inCytophaga hutchinsonii.Frontiers in Microbiology,2018, 9: 632-644

8. Xinfeng Bai, Xifeng Wang, Sen Wang, Xiaofei Ji, Zhiwei Guan, Weican Zhang andXuemei Lu*, Functional Studies of β-Glucosidases ofCytophaga hutchinsoniiand Their Effects on Cellulose Degradation,Frontiers in Microbiology,2017, 8:140. doi: 10.3389/fmicb.2017.00140

9.Sen Wang, Dong Zhao, Xinfeng Bai, Weican Zhang,Xuemei Lu*, Identification and Characterization of a Large Protein Essential for Degradation of the Crystalline Region of Cellulose byCytophaga hutchinsonii,Applied and Environmental Microbiology,2017, 83(1): e02270-16

10. Cong Zhang, Xifeng Wang, Weican Zhang, Yue Zhao,Xuemei Lu*, Expression and characterization of a glucose-tolerant β-1,4-glucosidase with wide substrate specificity fromCytophaga hutchinsonii,Applied Microbiology and Biotechnology,2017, 101(5): 1919–1926

11.Cong Zhang, Weican Zhang,Xuemei Lu*, Expression and characteristics of a Ca²⁺-dependent endoglucanase fromCytophaga hutchinsonii,Applied Microbiology and Biotechnology,2015, 99(22): 9617-9623

12. Zhe Li, Cong Zhang, Sen Wang, Jing Cao, Weican Zhang andXuemei Lu*,A new locus inCytophaga hutchinsoniiinvolved in colony spreading on agar surfaces and individual cell gliding,FEMS Microbiology Letters,2015, 362(14): 1-8

13.Ying Wang, Zhiquan Wang, Jing Cao, Zhiwei Guan andXuemei Lu*,FLP-FRT-based method to obtain unmarked deletions of CHU_3237 (porU) and large Genomic fragments ofCytophaga hutchinsonii,Applied and Environmental Microbiology,2014, 80 (19 ): 6037-6045

14.Xiaofei Ji, Ying Wang, Cong Zhang, Xinfeng Bai, Weican Zhang,Xuemei Lu*, A novel outer membrane protein involved in cellulose and cellooligosaccharide degradation byCytophaga hutchinsonii,Applied and Environmental Microbiology,2014, 80 (15): 4511-4518

15. Cong Zhang, Ying Wang, Zhe Li, Xiangru Zhou, Weican Zhang, Yue Zhao ,Xuemei Lu*, Characterization of a multi-function processive endoglucanase CHU_2103 fromCytophaga hutchinsonii,Applied Microbiology and Biotechnology,2014, 98 (15) : 6679-6687

获奖情况

1、国家科技奖自然科学二等奖（主要参与人）

2、山东省科技进步奖二等奖 （第一位）

3、山东省高等学校优秀科研成果奖二等奖（第一位）

4、山东大学教育拓展优秀名师

所获专利

1. 一株产γ-癸内酯的滴孔菌及其应用, 专利号：ZL 2017 1 0486577.1

2.一株产三萜类化合物的滴孔菌及其应用, 专利号：ZL 2017 1 0486576.7

3.一种制备交联剩余污泥重金属吸附剂的方法，专利号：ZL2014 1 0018162.8

4. 一种多功能纤维素酶及其表达基因与应用，专利号：ZL 201210439217.3

5. 大孔交联海藻酸钠凝胶球的制备方法，专利号：ZL 2011 1 0109497.7

6. 产β-1，3-葡聚糖酶的微生物及应用, 专利号：ZL 2009 1 0020307.7