教育背景

博士 1997-2001 德国明斯特大学 分子微生物

硕士 1989-1992 山东大学 微生物学

本科 1985-1989 山东大学 微生物学

工作经历

起止时间      单位名称         专业技术职务

2001-2003 德国克姆尼茨大学       博士后

2003 – 今  山东大学              教授

研究方向

1. 微生物代谢工程。研究代谢工程或者发酵过程中微生物的调节机制，利用代谢工程改造微生物生产高附加值产品或者化学品

2. 合成生物学。研究和开发合成生物学的方法和工具，探索微生物的生物合成理论与进化过程

科研项目

2018-2022，国家自然基金重点项目，利用非同源末端连接的基因组精简策略构建最适细胞工厂

2017-2020，国家自然基金面上项目，植物乳杆菌UDP-GlcNAc代谢调控机制及在N-乙酰神经氨酸合成中的应用

2015-2018，国家自然基金面上项目，大肠杆菌合成5-氨基乙酰丙酸的调控机制研究

2017-2019，山东省自然基金重大项目，结合非同源末端连接和同源重组的基因组编辑及其在细胞工厂中的应用

2016-2019，山东省科技发展重大项目，基于CRISPR重组的工业微生物改造技术及其应用

2014-2017，山东省自主创新重大转化项目，新型发酵产品的微生物制造关键技术

代表性论文

（2011年以来主要代表论文）

Peng Yang, Jing Wang, Qingxiao Pang, Fengyu Zhang, Junshu Wang, Qian Wang, Qingsheng Qi:Pathway optimization and key enzyme evolution of N -acetylneuraminate biosynthesis using an in vivo aptazyme-based biosensor. Metabolic Engineering 08/2017; 43., DOI:10.1016/j.ymben.2017.08.001

Zhiyong Cui, Cuijuan Gao, Jiaojiao Li, Jin Hou, Carol Sze Ki Lin, Qingsheng Qi:Engineering of unconventional yeast Yarrowia lipolytica for efficient succinic acid production from glycerol at low pH. Metabolic Engineering 06/2017; 42., DOI:10.1016/j.ymben.2017.06.007

Xinyuan He, Yan Chen, Quanfeng Liang, Qingsheng QI:An autoinduced AND-gate controlling metabolic pathway dynamically in response to microbial communities and cell physiological state. ACS Synthetic Biology 12/2016; 6(3)., DOI:10.1021/acssynbio.6b00177

Tianyuan Su, Fapeng Liu, Pengfei Gu, Haiying Jin, Yizhao Chang, Qian Wang, Quanfeng Liang, Qingsheng Qi:A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome. Scientific Reports 11/2016; 6:37895., DOI:10.1038/srep37895

Yizhao Chang, Tianyuan Su, Qingsheng Qi, Quanfeng Liang:Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system. Microbial Cell Factories 11/2016; 15(1)., DOI:10.1186/s12934-016-0594-4

Cuijuan Gao, Xiaofeng Yang, Huaimin Wang, Cristina Perez Rivero, Chong Li, Zhiyong Cui, Qingsheng Qi, Carol Sze Ki Lin:Robust succinic acid production from crude glycerol using engineered Yarrowia lipolytica. Biotechnology for Biofuels 08/2016; 9(1):179., DOI:10.1186/s13068-016-0597-8

Pengfei Gu, Tianyuan Su, Qian Wang, Quanfeng Liang, Qingsheng Qi:Tunable switch mediated shikimate biosynthesis in an engineered non-auxotrophic Escherichia coli. Scientific Reports 07/2016; 6:29745., DOI:10.1038/srep29745

Dongfang Gao, Yaqi Luan, Quanfeng Liang, Qingsheng Qi:Exploring the N-terminal role of a heterologous protein in secreting out of Escherichia coli: A non-classical signal peptide in protein secretion. Biotechnology and Bioengineering 06/2016; 113(12)., DOI:10.1002/bit.26028

Peng Yang, Wenjing Liu, Xuelian Cheng, Jing Wang, Qian Wang, Qingsheng Qi:A new strategy for the production of 5-aminolevulinic acid in recombinant Corynebacterium glutamicum with high yield. Applied and Environmental Microbiology 02/2016; 82(9)., DOI:10.1128/AEM.00224-16

Xiaoli Yu, Haiying Jin, Wenjing Liu, Qian Wang, Qingsheng Qi:Engineering Corynebacterium glutamicum to produce 5-aminolevulinic acid from glucose. Microbial Cell Factories 11/2015; 14(1):183., DOI:10.1186/s12934-015-0364-8

Dongfang Gao, Yaqi Luan, Qian Wang, Quanfeng Liang, Qingsheng Qi:Construction of cellulose-utilizing Escherichia coli based on a secretable cellulase. Microbial Cell Factories 10/2015; 14(1):159., DOI:10.1186/s12934-015-0349-7

Peng Yang, Jing Wang, Qingsheng Qi:Prophage recombinases-mediated genome engineering in Lactobacillus plantarum. Microbial Cell Factories 10/2015; 14(1)., DOI:10.1186/s12934-015-0344-z

Yongzhen Xia, Wenqiao Chu, Qingsheng Qi, Luying Xun:New insights into the QuikChangeTM process guide the use of Phusion DNA polymerase for site-directed mutagenesis. Nucleic Acids Research 11/2014; 43(2)., DOI:10.1093/nar/gku1189

Quanfeng Liang, Qingsheng Qi:From a co-production design to an integrated single-cell biorefinery. Biotechnology Advances 11/2014; 32(7)., DOI:10.1016/j.biotechadv.2014.08.004

Qianqian Zhuang, Qian Wang, Quanfeng Liang, Qingsheng Qi:Synthesis of polyhydroxyalkanoates from glucose that contain medium-chain-length monomers via the reversed fatty acid β-oxidation cycle in Escherichia coli. Metabolic Engineering 05/2014; 24., DOI:10.1016/j.ymben.2014.05.004

获奖情况

教育部新世纪优秀人才

山东省优秀博士论文指导教师

山东生博士生优秀成科研果指导教师

所获专利

1. 一株用于好氧发酵的大肠杆菌工程菌株ZL200910015399.X

2. 一种糖酰胺酶的蛋白及其编码序列与应用ZL200910229995.8

3. 利用生物柴油副产物生产琥珀酸和聚羟基脂肪酸酯的方法ZL201010605653.4

4. 一株重组大肠杆菌及其在生产5-氨基乙酰丙酸中的应用ZL201110079886.X

5. 一种重组大肠杆菌及应用其以单一碳源生产PHBV的方法201110079896.3

6. 一种纤维素酶在大肠杆菌中可溶及分泌表达重组蛋白的应用201210264387.2

7. 一种大肠杆菌工程菌株及其低氧发酵生产琥珀酸的应用ZL201210349656.5

8. 一种利用酵母生产β-环糊精的方法 ZL200610042071.3

9. 利用重组大肠杆菌联产琥珀酸和聚β羟基丁酸酯的方法ZL200810138886.0