教育背景
博士：2000.09-2004.03，中国科学院生物物理研究所，生物化学与分子生物学
硕士：1997.09-2000.07，四川大学，遗传学
学士：1993.09-1997.07，四川大学，微生物学
工作经历
2024.01-至今，山东大学微生物技术国家重点实验室，教授
2009.04-2023.12，中科院青岛生物能源与过程研究所，研究员
2008.01-2009.03，美国威斯康星大学麦迪逊分校，生化系，助理研究员
2004.08-2007.12，美国威斯康星大学麦迪逊分校，生化系，博士后
研究方向
1.非粮生物质的高效降解利用与秸秆糖平台
2.蛋白及脂类的先进生物制造
3.生物-化工融合工艺、自动化装备及智能优化算法
二、科研项目
主要项目
1. 2023.08-2028.07，新一代秸秆高效糖化利用技术，山东大学微生物技术国家重点实验室揭榜挂帅项目SKLMTFCP-2023-04，主持
2. 2022.01-2025.12，木质纤维素糖化过程中的纤维小体自适应调控机制及优化，国家自然科学基金面上项目，32170051，主持
3. 2021.10-2024.01，间歇式CBS秸秆糖化中试示范，山东省能源研究院专项基金，SEIS202106，主持
4. 2020.05-2022.04，青岛生物智造GMP公共服务平台，青岛市区局会商专项，20-3-1-7-CG，主持
5. 2018.06-2020.06，纤维素乙醇关键技术示范，中科院重点培育项目，Y87230190G，主持
6. 2018.01-2020.12，重要功能脂质分子的合成途径设计及细胞工厂构建，山东省重大基础研究项目，ZR2018ZB0208，主持
7. 2018.07-2023.07，秸秆清洁预处理及一锅法糖化关键技术，中科院战略性先导科技专项（A类）子课题，XDA21060201，主持
8. 2016.06-2021.05，近海生态系统碳汇形成过程与机理，国家重点研发计划课题，2016YFA0601402，主持
9. 2015.01-2018.12，热纤梭菌纤维小体的产物抑制机理及工程改造，国家自然科学基金面上项目，31470210，主持
10. 2011.01-2015.12，细菌酶复合体纤维素降解转化机理和纤维小体的解析重构，科技部973计划课题，2011CB707404，主持
11. 2011.01-2013.12，基于超声空化及电穿孔联合作用的通用细胞基因转移系统，中科院科研装备研制项目，YZ201138，主持
三、学术论文
代表性论文
2024
1.Huan Liu#*, Wei Gao#, Tianlun Cui, Sen Wang, Xiaojin Song, Zhuojun Wang, Huidan Zhang, Shiming Li, Yong-Liang Yu, Qiu Cui*(2024) A high-throughput platform enables in situ screening of fatty acid-producing strains using laser ablation electrospray ionization mass spectrometry and a Python package.Talanta268:125234.
2023
1. Huidan Zhang, Zhuojun Wang, Caili Sun, Chuchu Zhang, Huan Liu, Qiu Cui, Xiaojin Song* and Sen Wang*（2023）A phospholipid:diacylglycerol acyltransferase is involved in the regulation of phospholipids homeostasis in oleaginous Aurantiochytrium sp.Biotechnology for Biofuels and Bioproducts16:142.
2.刘欢，崔球* (2023)原位电离质谱技术在微生物菌株筛选中的应用进展，合成生物学4(5):980-999.
3.肖艳，刘亚君，冯银刚*，崔球*热纤梭菌在生物质能源开发中的合成生物学研究进展，合成生物学4(XX):1-26.
4. Jie Li, Haonan Zhang, Dongyu Li, Ya-Jun Liu, Edward A. Bayer, Qi Cui, Yingang Feng*, Ping Zhu* (2023) Structure of the transcription open complex of distinct σI factors.Nature Communications14:6455.
5. Chao Chen#, Bo Ma#, Yefei Wang#*, Qiu Cui, Lishan Yao, Yaohao Li, Baoquan Chen, Yingang Feng*, Zhongping Tan* (2023) Structural insight into why S-linked glycosylation cannot adequately mimic the role of natural O-glycosylation.Int. J. Biol. Macromol.253:126649.
6. Huidan Zhang, Weijian Wan, Qiu Cui*, Xiaojin Song* (2023) Modular metabolic engineering ofMortierella alpinaby the 2A peptide platform to improve arachidonic acid production.J. Agric. Food Chem.71(33):12519-12527.
7. Yan Xiao, Sheng Dong, Ya-Jun Liu, Chun You, Yingang Feng*, Qiu Cui* (2023) Key roles of β-glucosidase BglA for the catabolism of both laminaribiose and cellobiose in the lignocellulolytic bacterium Clostridium thermocellum. Int. J. Biol. Macromol. 250:126226.
8. Chao Chen, Sheng Dong, Zhaoli Yu, Yichen Qiao, Jie Li, Xiaoke Ding, Renmin Li, Jinzhong Lin, Edward A. Bayer, Ya-Jun Liu, Qiu Cui, Yingang Feng* (2023) Essential autoproteolysis of bacterial anti-σ factor RsgI for transmembrane signal transduction. Sci. Adv. 9(27):eadg4846.
9. Ya-Jun Liu, Yuedong Zhang, Fang Chi, Chaoyang Chen, Weijian Wan, Yingang Feng, Xiaojin Song, Qiu Cui* (2023) Integrated lactic acid production from lignocellulosic agricultural wastes under thermal conditions. J. Environ. Manage. 342:118281.
10. Cai You, Ya-Jun Liu, Qiu Cui*, Yingang Feng* (2023) Glycoside hydrolase family 48 cellulase: A key player in cellulolytic bacteria for lignocellulose biorefinery.Fermentation9(3):204.
11. Guang-Lei Liu, Xian-Ying Bu, Chaoyang Chen, Chunxiang Fu, Zhe Chi, Akihiko Kosugi, Qiu Cui, Zhen-Ming Chi, Ya-Jun Liu* (2023) Bioconversion of non-food corn biomass to polyol esters of fatty acid and single-cell oils. Biotechnol. Biofuels Bioprod. 16:9.
2022
1. Fei Yan#, Sheng Dong#, Ya-Jun Liu#, Xingzhe Yao, Chao Chen, Yan Xiao, Edward A. Bayer, Yuval Shoham, Chun You, Qiu Cui*, Yingang Feng* (2022) Deciphering cellodextrin and glucose uptake inClostridium thermocellum.mBio13(5):e01476-22.
2.颜飞，董维亮，崔球*，刘亚君*（2022）不可降解塑料的全细胞催化降解及升级再造。生物加工过程20(4):416-427.
3.肖艳，王璐，王森，丛培虎，陆栋，冯银刚，崔球，宋晓金*(2022)碳离子束诱变选育高产角鲨烯类酵母及电转化初探。南方水产科学18(2): 98-104.
4. Chao Chen, Kuan Qi, Fang Chi, Xiaojin Song, Yingang Feng, Qiu Cui, Ya-Jun Liu* (2022) Dissolved xylan inhibits cellulosome-based saccharification by binding to the key cellulosomal component ofClostridium thermocellum.Int. J. Biol. Macromol.207: 784-790.
5.冯银刚*，刘亚君，崔球（2022）纤维小体在合成生物学中的应用研究进展。合成生物学3(1):138-154.（综述）
2021
1. Sen Wang, Weijian Wan, Zhuojun Wang, Huidan Zhang, Huan Liu, K K I U Arunakumara, Qiu Cui, Xiaojin Song* (2021) A two-stage adaptive laboratory evolution strategy to enhance docosahexaenoic acid synthesis in oleaginous Thraustochytrid.Front. Nutr.8:795491.
2. Chuanzeng Lan#, Sen Wang#, Huidan Zhang, Zhuojun Wang, Weijian Wan, Huan Liu, Yang Hu, Qiu Cui, Xiaojin Song* (2021) Cocktail biosynthesis of triacylglycerol by rational modulation of diacylglycerol acyltransferases in industrial oleaginousAurantiochytrium.Biotechnol. Biofuels14(1):246.
3. Zhuojun Wang, Sen Wang*, Yingang Feng, Weijian Wan, Huidan Zhang, Xinfeng Bai, Qiu Cui, Xiaojin Song* (2021) Obtaining high-purity docosahexaenoic acid oil in ThraustochytridAurantiochytriumthrough a combined metabolic engineering strategy.J. Agric. Food. Chem.69(35):10215-10222.
4. Kuan Qi, Chao Chen, Fei Yan, Yingang Feng, Edward A. Bayer, Akihiko Kosugih, Qiu Cui, Ya-Jun Liu* (2021) Coordinated β-glucosidase activity with the cellulosome is effective for enhanced lignocellulose saccharification.Bioresour. Technol.337: 125441.
5. Jie Li, Chao Chen, Ya-Jun Liu, Qiu Cui, Edward A. Bayer, Yingang Feng* (2021) NMR chemical shift assignments of a module of unknown function in the cellulosomal secondary scaffoldin ScaF fromClostridium thermocellum.Biomol. NMR Assign.15(2):329-334.
6. Huidan Zhang, Qiu Cui, Xiaojin Song* (2021) Research advances on arachidonic acid production by fermentation and genetic modification ofMortierella alpina.World J. Microbiol. Biotechnol.37(1):4.
7. Sheng Dong, Ya-Jun Liu, Haixia Zhou, Yan Xiao, Jian Xu, Qiu Cui*, Xinquan Wang*, Yingang Feng* (2021) Structural insight into a GH1 β-glucosidase from the oleaginous microalga,Nannochloropsis oceanica.Int. J. Biol. Macromol.170:196-206.
8. Fei Yan, Ren Wei*, Qiu Cui*, Uwe T. Bornscheuer, Ya-Jun Liu* (2021) Thermophilic whole-cell degradation of polyethylene terephthalate using engineeredClostridium thermocellum.Microbial Biotechnol.14(2):374-385.
2020
1. Xingzhe Yao†, Chao Chen†, Yefei Wang†, Sheng Dong, Ya-Jun Liu, Yifei Li, Zhenling Cui, Weibin Gong, Sarah Perrett, Lishan Yao, Raphael Lamed, Edward A. Bayer, Qiu Cui, Yingang Feng* (2020) Discovery and mechanism of a pH-dependent dual-binding-site switch in the interaction of a pair of protein modules.Sci. Adv.6(43):eabd7182.
2. Sen Wang†, Chuanzeng Lan†, Zhuojun Wang, Weijian Wan, Huidan Zhang, Qiu Cui*, Xiaojin Song* (2020) Optimizing eicosapentaenoic acid production by grafting a heterologous polyketide synthase pathway in the ThraustochytridAurantiochytrium.J. Agric. Food Chem.68(40):11253-11260.
3. Sen Wang†, Chuanzeng Lan†, Zhuojun Wang, Weijian Wan, Qiu Cui*, Xiaojin Song* (2020) PUFA-synthase-specific PPTase enhanced the polyunsaturated fatty acid biosynthesis via the polyketide synthase pathway inAurantiochytrium.Biotechnol. Biofuels.13:152.
4. Jie Yang, Xiaojin Song, Lina Wang*, Qiu Cui* (2020) Comprehensive analysis of metabolic alterations inSchizochytriumsp. strains with different DHA content.J. Chromatog. B1160:122193.
5. Guanglei Liu, Xiaoxue Zhao, Chao Chen, Zhe Chi, Yuedong Zhang, Qiu Cui, Zhenming Chi, Ya-Jun Liu* (2020)Robust production of pigment-free pullulan from lignocellulosic hydrolysate by a new fungus co-utilizing glucose and xylose.Carbohydr. Polym.241:116400.
6. Ya-Jun Liu*, Bin Li, Yingang Feng*, Qiu Cui* (2020) Consolidated bio-saccharification: Leading lignocellulose bioconversion into the real world.Biotechnol. Adv.40:107535.
2019
1. Huidan Zhang, Zhuojun Wang, Yingang Feng, Qiu Cui*, Xiaojin Song* (2019) Phytohormones as stimulators to improve arachidonic acid biosynthesis inMortierella alpine.Enzyme Microbial Technol.131:109381.
2. Yanchao Wang, Xiaojin Song, Yingang Feng, Qiu Cui* (2019) Changes in peptidomes and Fischer ratios of corn-derived oligopeptides depending on enzyme hydrolysis approaches.Food Chem.297:124931.
3. Zhen Wei, Chao Chen, Ya-Jun Liu, Sheng Dong, Jie Li, Kuan Qi, Shiyue Liu, Xiaoke Ding, Lizett Ortiz de Ora, Iván Muñoz-Gutiérrez, Yifei Li, Hongwei Yao, Raphael Lamed, Edward A. Bayer, Qiu Cui, Yingang Feng* (2019) Alternative σI/anti-σIfactors represent a unique form of bacterial σ/anti-σ complex.Nucleic Acids Res.47(11):5988-5997.
4. Guzhen Cui†, Zhuojun Wang†, Wei Hong, Ya-Jun Liu, Zhenghong Chen, Qiu Cui*, Xiaojin Song* (2019) Enhancing tricarboxylate transportation-related NADPH generation to improve biodiesel production byAurantiochytrium.Algal Res.40:101505.
5. Xingzhe Yao, Ya-Jun Liu, Qiu Cui, Yingang Feng* (2019) Solution structure of a unicellular microalgae-derived translationally controlled tumor protein revealed both conserved features and structural diversity.Arch. Biochem. Biophys.665(1):23-29.
6. Shiyue Liu†, Ya-Jun Liu†, Yingang Feng, Bin Li and Qiu Cui* (2019) Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization.Biotechnol. Biofuels12:35.
7. Chao Chen, Hongwu Yang, Jinsong Xuan, Qiu Cui, Yingang Feng* (2019) Resonance assignments of a cellulosomal double-dockerin from Clostridium thermocellum. Biomol. NMR Assign. 13(1):97-101.
所获专利（近五年）
1.采用氧气处理方法的木质纤维素全细胞糖化技术；申请号CN202210645875.1；崔球，刘亚君，陈朝阳，宋晓金
2.原位分析微生物单菌落的激光电喷雾质谱装置；申请号202011006611.9；崔球，刘欢，宋晓金，王森，王卓君，蓝传增；2021.5.18授权
3.原位分析微生物菌落中角鲨烯含量的方法；申请号202011006613.8；宋晓金，刘欢，崔球，王森，张慧丹，万伟建，蓝传增；2021.8.17授权
4. PET降解生物催化剂及其应用；申请号202010013024.6；崔球，刘亚君，颜飞，韦韧；2021.12.31授权
5.富含均衡不饱和脂肪酸的橄榄油衍生物的制备及应用；申请号201910519311.1；崔球，邓煜，宋晓金，刘婷；2022.3.15授权
6.改善鸡蛋蛋壳质量的饲料及养殖方法；申请号201910366029.4；崔球，刘冰，宋晓金，程琦，周晓峰，王彦超；2021.11.19授权
7.一种低成本，高生物量和高蛋白量的蛋白核小球藻培养方法；申请号201811569914.4；崔球，肖艳，颜飞，祁宽；2022.4.15授权
8.用于生产高DHA含量鸡蛋的饲料添加剂及应用；申请号201811285475.4；崔球，宋晓金，刘亚君，蓝传僧，王森
9.用于生产适合婴幼儿的DHA-ARA鸡蛋的营养强化剂及应用；申请号201811282278.7；崔球，初欢欢，宋晓金，刘亚君，张慧丹；2021.4.13授权
10.一种无抗生物鸡饲料的制备方法；申请号201811277537.7；崔球，刘亚君，刘世岳，李仁民
11.生物饲料用促消化复合菌剂及其应用；发明专利号201811276681.9；崔球，初欢欢，刘亚君，刘世岳，李仁民；2020.10.20授权
12.采用木质纤维素生产纤维寡糖的方法；发明专利号201810985861.8；崔球，刘亚君，李仁民，冯银刚；2021.1.1授权
13.用于催化木质纤维素糖化的全菌酶制剂；申请号201810939329.2；崔球，刘亚君，刘世岳，李仁民，祁宽，冯银刚；2021.4.13授权
14.提高木质纤维素糖化效率的全菌糖化方法；发明专利号201810939479.3；崔球，刘亚君，刘世岳，冯银刚；2020.10.20授权
15.采用木质纤维素制备角鲨烯的方法；申请号201810939517.5；崔球，刘亚君，宋晓金；2021.4.27授权
16.采用木质纤维素制备虾青素的方法；申请号201810939518.X；崔球，刘亚君，宋晓金；2021.4.27授权
17.用于催化木质纤维素糖化的纤维小体酶制剂；申请号201810939532.X；崔球，刘亚君，李仁民，刘世岳，祁宽，冯银刚；2021.4.13授权
18.采用木质纤维素培养微藻的方法；申请号201810939294.2；崔球，刘亚君，祁宽，宋晓金；2021.9.28授权
19.采用木质纤维素生产富含多不饱和脂肪酸油脂的方法；申请号201810939296.1；崔球，刘亚君，李仁民，宋晓金，张慧丹；2022.04.15
20.采用木质纤维素制备乙醇的方法；申请号201810939170.4；崔球，刘亚君，李滨，冯银刚；2022.04.15授权
21.采用木质纤维素制备葡萄糖酸钠的方法；申请号201810939181.2；崔球，刘亚君，祁宽，冯银刚；2021.4.27授权
22.采用木质纤维素制备DHA的方法；申请号201810939182.7；崔球，刘亚君，刘世岳，宋晓金；2021.10.1授权
23.木质纤维素一锅法生物转化方法；发明专利号201810939277.9；崔球，刘亚君，李滨，冯银刚；2020.10.20授权
技术转移转化
1.高效基因转化仪，青岛百奥新创生物技术有限公司
2.高通量菌株选育系统，青岛百奥新创生物技术有限公司
3. DHA单细胞油脂生产技术，青岛琅琊台集团
4. ARA单细胞油脂生产技术，青岛琅琊台集团
5.非粮生物质高效转化联产单细胞蛋白及油脂，山东寿光巨能金玉米开发有限公司
6.微生物油脂高效发酵生产技术，河南新拓洋生物工程有限公司
7.盐湖卤虫营养强化的单细胞蛋白饲料生产技术，山西南风化工集团股份有限公司
软件著作权
1．高通量基因转化系统控制平台，2023SR0808352
2．青能所代谢组学视觉预处理平台，2023SR0752423
3．青能云数据可视化分析平台，2023SR0752424
4．智能生物实验室深度学习视觉标注系统，2023SR0988017
5．智能实验室DNA序列编辑系统，2023SR0989011
6．智能液体处理系统控制平台，2023SR0808353
7．自动化生物实验室机器人集群控制平台，2023SR0808351
获奖情况
1. 2023.11，高产DHA海洋微藻的菌株优选及清洁生产示范，中国食品工业协会科学技术奖，排名第三
2. 2022.07，秸秆木质纤维清洁高效预处理关键技术及应用，中国石油和化工联合会技术发明三等奖，排名第三
3. 2017.03，高产ARA高产高山被孢霉的菌株选育及清洁产生示范，青岛市科技进步三等奖，排名第一
4. 2016.01，高产DHA海洋微藻的菌株选育及清洁生产示范项目，山东省科学技术奖，排名第一
