生物与医药

崔球

教授
博士/硕士生导师
个人简介 科研项目 学术论文 其他介绍

教育背景

博士:2000.09-2004.03,中国科学院生物物理研究所,生物化学与分子生物学

硕士:1997.09-2000.07,四川大学,遗传学

学士:1993.09-1997.07,四川大学,微生物学

工作经历

2024.01-至今,山东大学微生物技术国家重点实验室,教授

2009.04-2023.12,中科院青岛生物能源与过程研究所,研究员

2008.01-2009.03,美国威斯康星大学麦迪逊分校,生化系,助理研究员

2004.08-2007.12,美国威斯康星大学麦迪逊分校,生化系,博士后

研究方向

1.非粮生物质的高效降解利用与秸秆糖平台

2.蛋白及脂类的先进生物制造

3.生物-化工融合工艺、自动化装备及智能优化算法

主要项目

1. 2023.08-2028.07,新一代秸秆高效糖化利用技术,山东大学微生物技术国家重点实验室揭榜挂帅项目SKLMTFCP-2023-04,主持

2. 2022.01-2025.12,木质纤维素糖化过程中的纤维小体自适应调控机制及优化,国家自然科学基金面上项目,32170051,主持

3. 2021.10-2024.01,间歇式CBS秸秆糖化中试示范,山东省能源研究院专项基金,SEIS202106,主持

4. 2020.05-2022.04,青岛生物智造GMP公共服务平台,青岛市区局会商专项,20-3-1-7-CG,主持

5. 2018.06-2020.06,纤维素乙醇关键技术示范,中科院重点培育项目,Y87230190G,主持

6. 2018.01-2020.12,重要功能脂质分子的合成途径设计及细胞工厂构建,山东省重大基础研究项目,ZR2018ZB0208,主持

7. 2018.07-2023.07,秸秆清洁预处理及一锅法糖化关键技术,中科院战略性先导科技专项(A类)子课题,XDA21060201,主持

8. 2016.06-2021.05,近海生态系统碳汇形成过程与机理,国家重点研发计划课题,2016YFA0601402,主持

9. 2015.01-2018.12,热纤梭菌纤维小体的产物抑制机理及工程改造,国家自然科学基金面上项目,31470210,主持

10. 2011.01-2015.12,细菌酶复合体纤维素降解转化机理和纤维小体的解析重构,科技部973计划课题,2011CB707404,主持

11. 2011.01-2013.12,基于超声空化及电穿孔联合作用的通用细胞基因转移系统,中科院科研装备研制项目,YZ201138,主持

代表性论文

2024

1.Huan Liu#*, Wei Gao#, Tianlun Cui, Sen Wang, Xiaojin Song, Zhuojun Wang, Huidan Zhang, Shiming Li, Yong-Liang Yu, Qiu Cui*(2024) A high-throughput platform enables in situ screening of fatty acid-producing strains using laser ablation electrospray ionization mass spectrometry and a Python package.Talanta268:125234.

2023

1. Huidan Zhang, Zhuojun Wang, Caili Sun, Chuchu Zhang, Huan Liu, Qiu Cui, Xiaojin Song* and Sen Wang*(2023)A phospholipid:diacylglycerol acyltransferase is involved in the regulation of phospholipids homeostasis in oleaginous Aurantiochytrium sp.Biotechnology for Biofuels and Bioproducts16:142.

2.刘欢,崔球* (2023)原位电离质谱技术在微生物菌株筛选中的应用进展,合成生物学4(5):980-999.

3.肖艳,刘亚君,冯银刚*,崔球*热纤梭菌在生物质能源开发中的合成生物学研究进展,合成生物学4(XX):1-26.

4. Jie Li, Haonan Zhang, Dongyu Li, Ya-Jun Liu, Edward A. Bayer, Qi Cui, Yingang Feng*, Ping Zhu* (2023) Structure of the transcription open complex of distinct σI factors.Nature Communications14:6455.

5. Chao Chen#, Bo Ma#, Yefei Wang#*, Qiu Cui, Lishan Yao, Yaohao Li, Baoquan Chen, Yingang Feng*, Zhongping Tan* (2023) Structural insight into why S-linked glycosylation cannot adequately mimic the role of natural O-glycosylation.Int. J. Biol. Macromol.253:126649.

6. Huidan Zhang, Weijian Wan, Qiu Cui*, Xiaojin Song* (2023) Modular metabolic engineering ofMortierella alpinaby the 2A peptide platform to improve arachidonic acid production.J. Agric. Food Chem.71(33):12519-12527.

7. Yan Xiao, Sheng Dong, Ya-Jun Liu, Chun You, Yingang Feng*, Qiu Cui* (2023) Key roles of β-glucosidase BglA for the catabolism of both laminaribiose and cellobiose in the lignocellulolytic bacterium Clostridium thermocellum. Int. J. Biol. Macromol. 250:126226.

8. Chao Chen, Sheng Dong, Zhaoli Yu, Yichen Qiao, Jie Li, Xiaoke Ding, Renmin Li, Jinzhong Lin, Edward A. Bayer, Ya-Jun Liu, Qiu Cui, Yingang Feng* (2023) Essential autoproteolysis of bacterial anti-σ factor RsgI for transmembrane signal transduction. Sci. Adv. 9(27):eadg4846.

9. Ya-Jun Liu, Yuedong Zhang, Fang Chi, Chaoyang Chen, Weijian Wan, Yingang Feng, Xiaojin Song, Qiu Cui* (2023) Integrated lactic acid production from lignocellulosic agricultural wastes under thermal conditions. J. Environ. Manage. 342:118281.

10. Cai You, Ya-Jun Liu, Qiu Cui*, Yingang Feng* (2023) Glycoside hydrolase family 48 cellulase: A key player in cellulolytic bacteria for lignocellulose biorefinery.Fermentation9(3):204.

11. Guang-Lei Liu, Xian-Ying Bu, Chaoyang Chen, Chunxiang Fu, Zhe Chi, Akihiko Kosugi, Qiu Cui, Zhen-Ming Chi, Ya-Jun Liu* (2023) Bioconversion of non-food corn biomass to polyol esters of fatty acid and single-cell oils. Biotechnol. Biofuels Bioprod. 16:9.

2022

1. Fei Yan#, Sheng Dong#, Ya-Jun Liu#, Xingzhe Yao, Chao Chen, Yan Xiao, Edward A. Bayer, Yuval Shoham, Chun You, Qiu Cui*, Yingang Feng* (2022) Deciphering cellodextrin and glucose uptake inClostridium thermocellum.mBio13(5):e01476-22.

2.颜飞,董维亮,崔球*,刘亚君*(2022)不可降解塑料的全细胞催化降解及升级再造。生物加工过程20(4):416-427.

3.肖艳,王璐,王森,丛培虎,陆栋,冯银刚,崔球,宋晓金*(2022)碳离子束诱变选育高产角鲨烯类酵母及电转化初探。南方水产科学18(2): 98-104.

4. Chao Chen, Kuan Qi, Fang Chi, Xiaojin Song, Yingang Feng, Qiu Cui, Ya-Jun Liu* (2022) Dissolved xylan inhibits cellulosome-based saccharification by binding to the key cellulosomal component ofClostridium thermocellum.Int. J. Biol. Macromol.207: 784-790.

5.冯银刚*,刘亚君,崔球(2022)纤维小体在合成生物学中的应用研究进展。合成生物学3(1):138-154.(综述)

2021

1. Sen Wang, Weijian Wan, Zhuojun Wang, Huidan Zhang, Huan Liu, K K I U Arunakumara, Qiu Cui, Xiaojin Song* (2021) A two-stage adaptive laboratory evolution strategy to enhance docosahexaenoic acid synthesis in oleaginous Thraustochytrid.Front. Nutr.8:795491.

2. Chuanzeng Lan#, Sen Wang#, Huidan Zhang, Zhuojun Wang, Weijian Wan, Huan Liu, Yang Hu, Qiu Cui, Xiaojin Song* (2021) Cocktail biosynthesis of triacylglycerol by rational modulation of diacylglycerol acyltransferases in industrial oleaginousAurantiochytrium.Biotechnol. Biofuels14(1):246.

3. Zhuojun Wang, Sen Wang*, Yingang Feng, Weijian Wan, Huidan Zhang, Xinfeng Bai, Qiu Cui, Xiaojin Song* (2021) Obtaining high-purity docosahexaenoic acid oil in ThraustochytridAurantiochytriumthrough a combined metabolic engineering strategy.J. Agric. Food. Chem.69(35):10215-10222.

4. Kuan Qi, Chao Chen, Fei Yan, Yingang Feng, Edward A. Bayer, Akihiko Kosugih, Qiu Cui, Ya-Jun Liu* (2021) Coordinated β-glucosidase activity with the cellulosome is effective for enhanced lignocellulose saccharification.Bioresour. Technol.337: 125441.

5. Jie Li, Chao Chen, Ya-Jun Liu, Qiu Cui, Edward A. Bayer, Yingang Feng* (2021) NMR chemical shift assignments of a module of unknown function in the cellulosomal secondary scaffoldin ScaF fromClostridium thermocellum.Biomol. NMR Assign.15(2):329-334.

6. Huidan Zhang, Qiu Cui, Xiaojin Song* (2021) Research advances on arachidonic acid production by fermentation and genetic modification ofMortierella alpina.World J. Microbiol. Biotechnol.37(1):4.

7. Sheng Dong, Ya-Jun Liu, Haixia Zhou, Yan Xiao, Jian Xu, Qiu Cui*, Xinquan Wang*, Yingang Feng* (2021) Structural insight into a GH1 β-glucosidase from the oleaginous microalga,Nannochloropsis oceanica.Int. J. Biol. Macromol.170:196-206.

8. Fei Yan, Ren Wei*, Qiu Cui*, Uwe T. Bornscheuer, Ya-Jun Liu* (2021) Thermophilic whole-cell degradation of polyethylene terephthalate using engineeredClostridium thermocellum.Microbial Biotechnol.14(2):374-385.

2020

1. Xingzhe Yao†, Chao Chen†, Yefei Wang†, Sheng Dong, Ya-Jun Liu, Yifei Li, Zhenling Cui, Weibin Gong, Sarah Perrett, Lishan Yao, Raphael Lamed, Edward A. Bayer, Qiu Cui, Yingang Feng* (2020) Discovery and mechanism of a pH-dependent dual-binding-site switch in the interaction of a pair of protein modules.Sci. Adv.6(43):eabd7182.

2. Sen Wang†, Chuanzeng Lan†, Zhuojun Wang, Weijian Wan, Huidan Zhang, Qiu Cui*, Xiaojin Song* (2020) Optimizing eicosapentaenoic acid production by grafting a heterologous polyketide synthase pathway in the ThraustochytridAurantiochytrium.J. Agric. Food Chem.68(40):11253-11260.

3. Sen Wang†, Chuanzeng Lan†, Zhuojun Wang, Weijian Wan, Qiu Cui*, Xiaojin Song* (2020) PUFA-synthase-specific PPTase enhanced the polyunsaturated fatty acid biosynthesis via the polyketide synthase pathway inAurantiochytrium.Biotechnol. Biofuels.13:152.

4. Jie Yang, Xiaojin Song, Lina Wang*, Qiu Cui* (2020) Comprehensive analysis of metabolic alterations inSchizochytriumsp. strains with different DHA content.J. Chromatog. B1160:122193.

5. Guanglei Liu, Xiaoxue Zhao, Chao Chen, Zhe Chi, Yuedong Zhang, Qiu Cui, Zhenming Chi, Ya-Jun Liu* (2020)Robust production of pigment-free pullulan from lignocellulosic hydrolysate by a new fungus co-utilizing glucose and xylose.Carbohydr. Polym.241:116400.

6. Ya-Jun Liu*, Bin Li, Yingang Feng*, Qiu Cui* (2020) Consolidated bio-saccharification: Leading lignocellulose bioconversion into the real world.Biotechnol. Adv.40:107535.

2019

1. Huidan Zhang, Zhuojun Wang, Yingang Feng, Qiu Cui*, Xiaojin Song* (2019) Phytohormones as stimulators to improve arachidonic acid biosynthesis inMortierella alpine.Enzyme Microbial Technol.131:109381.

2. Yanchao Wang, Xiaojin Song, Yingang Feng, Qiu Cui* (2019) Changes in peptidomes and Fischer ratios of corn-derived oligopeptides depending on enzyme hydrolysis approaches.Food Chem.297:124931.

3. Zhen Wei, Chao Chen, Ya-Jun Liu, Sheng Dong, Jie Li, Kuan Qi, Shiyue Liu, Xiaoke Ding, Lizett Ortiz de Ora, Iván Muñoz-Gutiérrez, Yifei Li, Hongwei Yao, Raphael Lamed, Edward A. Bayer, Qiu Cui, Yingang Feng* (2019) Alternative σI/anti-σIfactors represent a unique form of bacterial σ/anti-σ complex.Nucleic Acids Res.47(11):5988-5997.

4. Guzhen Cui†, Zhuojun Wang†, Wei Hong, Ya-Jun Liu, Zhenghong Chen, Qiu Cui*, Xiaojin Song* (2019) Enhancing tricarboxylate transportation-related NADPH generation to improve biodiesel production byAurantiochytrium.Algal Res.40:101505.

5. Xingzhe Yao, Ya-Jun Liu, Qiu Cui, Yingang Feng* (2019) Solution structure of a unicellular microalgae-derived translationally controlled tumor protein revealed both conserved features and structural diversity.Arch. Biochem. Biophys.665(1):23-29.

6. Shiyue Liu†, Ya-Jun Liu†, Yingang Feng, Bin Li and Qiu Cui* (2019) Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization.Biotechnol. Biofuels12:35.

7. Chao Chen, Hongwu Yang, Jinsong Xuan, Qiu Cui, Yingang Feng* (2019) Resonance assignments of a cellulosomal double-dockerin from Clostridium thermocellum. Biomol. NMR Assign. 13(1):97-101.

所获专利(近五年)

1.采用氧气处理方法的木质纤维素全细胞糖化技术;申请号CN202210645875.1;崔球,刘亚君,陈朝阳,宋晓金

2.原位分析微生物单菌落的激光电喷雾质谱装置;申请号202011006611.9;崔球,刘欢,宋晓金,王森,王卓君,蓝传增;2021.5.18授权

3.原位分析微生物菌落中角鲨烯含量的方法;申请号202011006613.8;宋晓金,刘欢,崔球,王森,张慧丹,万伟建,蓝传增;2021.8.17授权

4. PET降解生物催化剂及其应用;申请号202010013024.6;崔球,刘亚君,颜飞,韦韧;2021.12.31授权

5.富含均衡不饱和脂肪酸的橄榄油衍生物的制备及应用;申请号201910519311.1;崔球,邓煜,宋晓金,刘婷;2022.3.15授权

6.改善鸡蛋蛋壳质量的饲料及养殖方法;申请号201910366029.4;崔球,刘冰,宋晓金,程琦,周晓峰,王彦超;2021.11.19授权

7.一种低成本,高生物量和高蛋白量的蛋白核小球藻培养方法;申请号201811569914.4;崔球,肖艳,颜飞,祁宽;2022.4.15授权

8.用于生产高DHA含量鸡蛋的饲料添加剂及应用;申请号201811285475.4;崔球,宋晓金,刘亚君,蓝传僧,王森

9.用于生产适合婴幼儿的DHA-ARA鸡蛋的营养强化剂及应用;申请号201811282278.7;崔球,初欢欢,宋晓金,刘亚君,张慧丹;2021.4.13授权

10.一种无抗生物鸡饲料的制备方法;申请号201811277537.7;崔球,刘亚君,刘世岳,李仁民

11.生物饲料用促消化复合菌剂及其应用;发明专利号201811276681.9;崔球,初欢欢,刘亚君,刘世岳,李仁民;2020.10.20授权

12.采用木质纤维素生产纤维寡糖的方法;发明专利号201810985861.8;崔球,刘亚君,李仁民,冯银刚;2021.1.1授权

13.用于催化木质纤维素糖化的全菌酶制剂;申请号201810939329.2;崔球,刘亚君,刘世岳,李仁民,祁宽,冯银刚;2021.4.13授权

14.提高木质纤维素糖化效率的全菌糖化方法;发明专利号201810939479.3;崔球,刘亚君,刘世岳,冯银刚;2020.10.20授权

15.采用木质纤维素制备角鲨烯的方法;申请号201810939517.5;崔球,刘亚君,宋晓金;2021.4.27授权

16.采用木质纤维素制备虾青素的方法;申请号201810939518.X;崔球,刘亚君,宋晓金;2021.4.27授权

17.用于催化木质纤维素糖化的纤维小体酶制剂;申请号201810939532.X;崔球,刘亚君,李仁民,刘世岳,祁宽,冯银刚;2021.4.13授权

18.采用木质纤维素培养微藻的方法;申请号201810939294.2;崔球,刘亚君,祁宽,宋晓金;2021.9.28授权

19.采用木质纤维素生产富含多不饱和脂肪酸油脂的方法;申请号201810939296.1;崔球,刘亚君,李仁民,宋晓金,张慧丹;2022.04.15

20.采用木质纤维素制备乙醇的方法;申请号201810939170.4;崔球,刘亚君,李滨,冯银刚;2022.04.15授权

21.采用木质纤维素制备葡萄糖酸钠的方法;申请号201810939181.2;崔球,刘亚君,祁宽,冯银刚;2021.4.27授权

22.采用木质纤维素制备DHA的方法;申请号201810939182.7;崔球,刘亚君,刘世岳,宋晓金;2021.10.1授权

23.木质纤维素一锅法生物转化方法;发明专利号201810939277.9;崔球,刘亚君,李滨,冯银刚;2020.10.20授权


技术转移转化

1.高效基因转化仪,青岛百奥新创生物技术有限公司

2.高通量菌株选育系统,青岛百奥新创生物技术有限公司

3. DHA单细胞油脂生产技术,青岛琅琊台集团

4. ARA单细胞油脂生产技术,青岛琅琊台集团

5.非粮生物质高效转化联产单细胞蛋白及油脂,山东寿光巨能金玉米开发有限公司

6.微生物油脂高效发酵生产技术,河南新拓洋生物工程有限公司

7.盐湖卤虫营养强化的单细胞蛋白饲料生产技术,山西南风化工集团股份有限公司


软件著作权

1.高通量基因转化系统控制平台,2023SR0808352

2.青能所代谢组学视觉预处理平台,2023SR0752423

3.青能云数据可视化分析平台,2023SR0752424

4.智能生物实验室深度学习视觉标注系统,2023SR0988017

5.智能实验室DNA序列编辑系统,2023SR0989011

6.智能液体处理系统控制平台,2023SR0808353

7.自动化生物实验室机器人集群控制平台,2023SR0808351


获奖情况

1. 2023.11,高产DHA海洋微藻的菌株优选及清洁生产示范,中国食品工业协会科学技术奖,排名第三

2. 2022.07,秸秆木质纤维清洁高效预处理关键技术及应用,中国石油和化工联合会技术发明三等奖,排名第三

3. 2017.03,高产ARA高产高山被孢霉的菌株选育及清洁产生示范,青岛市科技进步三等奖,排名第一

4. 2016.01,高产DHA海洋微藻的菌株选育及清洁生产示范项目,山东省科学技术奖,排名第一