代表性论文
2024
1.Huan Liu#*, Wei Gao#, Tianlun Cui, Sen Wang, Xiaojin Song, Zhuojun Wang, Huidan Zhang, Shiming Li, Yong-Liang Yu, Qiu Cui*(2024) A high-throughput platform enables in situ screening of fatty acid-producing strains using laser ablation electrospray ionization mass spectrometry and a Python package.Talanta268:125234.
2023
1. Huidan Zhang, Zhuojun Wang, Caili Sun, Chuchu Zhang, Huan Liu, Qiu Cui, Xiaojin Song* and Sen Wang*(2023)A phospholipid:diacylglycerol acyltransferase is involved in the regulation of phospholipids homeostasis in oleaginous Aurantiochytrium sp.Biotechnology for Biofuels and Bioproducts16:142.
2.刘欢,崔球* (2023)原位电离质谱技术在微生物菌株筛选中的应用进展,合成生物学4(5):980-999.
3.肖艳,刘亚君,冯银刚*,崔球*热纤梭菌在生物质能源开发中的合成生物学研究进展,合成生物学4(XX):1-26.
4. Jie Li, Haonan Zhang, Dongyu Li, Ya-Jun Liu, Edward A. Bayer, Qi Cui, Yingang Feng*, Ping Zhu* (2023) Structure of the transcription open complex of distinct σI factors.Nature Communications14:6455.
5. Chao Chen#, Bo Ma#, Yefei Wang#*, Qiu Cui, Lishan Yao, Yaohao Li, Baoquan Chen, Yingang Feng*, Zhongping Tan* (2023) Structural insight into why S-linked glycosylation cannot adequately mimic the role of natural O-glycosylation.Int. J. Biol. Macromol.253:126649.
6. Huidan Zhang, Weijian Wan, Qiu Cui*, Xiaojin Song* (2023) Modular metabolic engineering ofMortierella alpinaby the 2A peptide platform to improve arachidonic acid production.J. Agric. Food Chem.71(33):12519-12527.
7. Yan Xiao, Sheng Dong, Ya-Jun Liu, Chun You, Yingang Feng*, Qiu Cui* (2023) Key roles of β-glucosidase BglA for the catabolism of both laminaribiose and cellobiose in the lignocellulolytic bacterium Clostridium thermocellum. Int. J. Biol. Macromol. 250:126226.
8. Chao Chen, Sheng Dong, Zhaoli Yu, Yichen Qiao, Jie Li, Xiaoke Ding, Renmin Li, Jinzhong Lin, Edward A. Bayer, Ya-Jun Liu, Qiu Cui, Yingang Feng* (2023) Essential autoproteolysis of bacterial anti-σ factor RsgI for transmembrane signal transduction. Sci. Adv. 9(27):eadg4846.
9. Ya-Jun Liu, Yuedong Zhang, Fang Chi, Chaoyang Chen, Weijian Wan, Yingang Feng, Xiaojin Song, Qiu Cui* (2023) Integrated lactic acid production from lignocellulosic agricultural wastes under thermal conditions. J. Environ. Manage. 342:118281.
10. Cai You, Ya-Jun Liu, Qiu Cui*, Yingang Feng* (2023) Glycoside hydrolase family 48 cellulase: A key player in cellulolytic bacteria for lignocellulose biorefinery.Fermentation9(3):204.
11. Guang-Lei Liu, Xian-Ying Bu, Chaoyang Chen, Chunxiang Fu, Zhe Chi, Akihiko Kosugi, Qiu Cui, Zhen-Ming Chi, Ya-Jun Liu* (2023) Bioconversion of non-food corn biomass to polyol esters of fatty acid and single-cell oils. Biotechnol. Biofuels Bioprod. 16:9.
2022
1. Fei Yan#, Sheng Dong#, Ya-Jun Liu#, Xingzhe Yao, Chao Chen, Yan Xiao, Edward A. Bayer, Yuval Shoham, Chun You, Qiu Cui*, Yingang Feng* (2022) Deciphering cellodextrin and glucose uptake inClostridium thermocellum.mBio13(5):e01476-22.
2.颜飞,董维亮,崔球*,刘亚君*(2022)不可降解塑料的全细胞催化降解及升级再造。生物加工过程20(4):416-427.
3.肖艳,王璐,王森,丛培虎,陆栋,冯银刚,崔球,宋晓金*(2022)碳离子束诱变选育高产角鲨烯类酵母及电转化初探。南方水产科学18(2): 98-104.
4. Chao Chen, Kuan Qi, Fang Chi, Xiaojin Song, Yingang Feng, Qiu Cui, Ya-Jun Liu* (2022) Dissolved xylan inhibits cellulosome-based saccharification by binding to the key cellulosomal component ofClostridium thermocellum.Int. J. Biol. Macromol.207: 784-790.
5.冯银刚*,刘亚君,崔球(2022)纤维小体在合成生物学中的应用研究进展。合成生物学3(1):138-154.(综述)
2021
1. Sen Wang, Weijian Wan, Zhuojun Wang, Huidan Zhang, Huan Liu, K K I U Arunakumara, Qiu Cui, Xiaojin Song* (2021) A two-stage adaptive laboratory evolution strategy to enhance docosahexaenoic acid synthesis in oleaginous Thraustochytrid.Front. Nutr.8:795491.
2. Chuanzeng Lan#, Sen Wang#, Huidan Zhang, Zhuojun Wang, Weijian Wan, Huan Liu, Yang Hu, Qiu Cui, Xiaojin Song* (2021) Cocktail biosynthesis of triacylglycerol by rational modulation of diacylglycerol acyltransferases in industrial oleaginousAurantiochytrium.Biotechnol. Biofuels14(1):246.
3. Zhuojun Wang, Sen Wang*, Yingang Feng, Weijian Wan, Huidan Zhang, Xinfeng Bai, Qiu Cui, Xiaojin Song* (2021) Obtaining high-purity docosahexaenoic acid oil in ThraustochytridAurantiochytriumthrough a combined metabolic engineering strategy.J. Agric. Food. Chem.69(35):10215-10222.
4. Kuan Qi, Chao Chen, Fei Yan, Yingang Feng, Edward A. Bayer, Akihiko Kosugih, Qiu Cui, Ya-Jun Liu* (2021) Coordinated β-glucosidase activity with the cellulosome is effective for enhanced lignocellulose saccharification.Bioresour. Technol.337: 125441.
5. Jie Li, Chao Chen, Ya-Jun Liu, Qiu Cui, Edward A. Bayer, Yingang Feng* (2021) NMR chemical shift assignments of a module of unknown function in the cellulosomal secondary scaffoldin ScaF fromClostridium thermocellum.Biomol. NMR Assign.15(2):329-334.
6. Huidan Zhang, Qiu Cui, Xiaojin Song* (2021) Research advances on arachidonic acid production by fermentation and genetic modification ofMortierella alpina.World J. Microbiol. Biotechnol.37(1):4.
7. Sheng Dong, Ya-Jun Liu, Haixia Zhou, Yan Xiao, Jian Xu, Qiu Cui*, Xinquan Wang*, Yingang Feng* (2021) Structural insight into a GH1 β-glucosidase from the oleaginous microalga,Nannochloropsis oceanica.Int. J. Biol. Macromol.170:196-206.
8. Fei Yan, Ren Wei*, Qiu Cui*, Uwe T. Bornscheuer, Ya-Jun Liu* (2021) Thermophilic whole-cell degradation of polyethylene terephthalate using engineeredClostridium thermocellum.Microbial Biotechnol.14(2):374-385.
2020
1. Xingzhe Yao†, Chao Chen†, Yefei Wang†, Sheng Dong, Ya-Jun Liu, Yifei Li, Zhenling Cui, Weibin Gong, Sarah Perrett, Lishan Yao, Raphael Lamed, Edward A. Bayer, Qiu Cui, Yingang Feng* (2020) Discovery and mechanism of a pH-dependent dual-binding-site switch in the interaction of a pair of protein modules.Sci. Adv.6(43):eabd7182.
2. Sen Wang†, Chuanzeng Lan†, Zhuojun Wang, Weijian Wan, Huidan Zhang, Qiu Cui*, Xiaojin Song* (2020) Optimizing eicosapentaenoic acid production by grafting a heterologous polyketide synthase pathway in the ThraustochytridAurantiochytrium.J. Agric. Food Chem.68(40):11253-11260.
3. Sen Wang†, Chuanzeng Lan†, Zhuojun Wang, Weijian Wan, Qiu Cui*, Xiaojin Song* (2020) PUFA-synthase-specific PPTase enhanced the polyunsaturated fatty acid biosynthesis via the polyketide synthase pathway inAurantiochytrium.Biotechnol. Biofuels.13:152.
4. Jie Yang, Xiaojin Song, Lina Wang*, Qiu Cui* (2020) Comprehensive analysis of metabolic alterations inSchizochytriumsp. strains with different DHA content.J. Chromatog. B1160:122193.
5. Guanglei Liu, Xiaoxue Zhao, Chao Chen, Zhe Chi, Yuedong Zhang, Qiu Cui, Zhenming Chi, Ya-Jun Liu* (2020)Robust production of pigment-free pullulan from lignocellulosic hydrolysate by a new fungus co-utilizing glucose and xylose.Carbohydr. Polym.241:116400.
6. Ya-Jun Liu*, Bin Li, Yingang Feng*, Qiu Cui* (2020) Consolidated bio-saccharification: Leading lignocellulose bioconversion into the real world.Biotechnol. Adv.40:107535.
2019
1. Huidan Zhang, Zhuojun Wang, Yingang Feng, Qiu Cui*, Xiaojin Song* (2019) Phytohormones as stimulators to improve arachidonic acid biosynthesis inMortierella alpine.Enzyme Microbial Technol.131:109381.
2. Yanchao Wang, Xiaojin Song, Yingang Feng, Qiu Cui* (2019) Changes in peptidomes and Fischer ratios of corn-derived oligopeptides depending on enzyme hydrolysis approaches.Food Chem.297:124931.
3. Zhen Wei, Chao Chen, Ya-Jun Liu, Sheng Dong, Jie Li, Kuan Qi, Shiyue Liu, Xiaoke Ding, Lizett Ortiz de Ora, Iván Muñoz-Gutiérrez, Yifei Li, Hongwei Yao, Raphael Lamed, Edward A. Bayer, Qiu Cui, Yingang Feng* (2019) Alternative σI/anti-σIfactors represent a unique form of bacterial σ/anti-σ complex.Nucleic Acids Res.47(11):5988-5997.
4. Guzhen Cui†, Zhuojun Wang†, Wei Hong, Ya-Jun Liu, Zhenghong Chen, Qiu Cui*, Xiaojin Song* (2019) Enhancing tricarboxylate transportation-related NADPH generation to improve biodiesel production byAurantiochytrium.Algal Res.40:101505.
5. Xingzhe Yao, Ya-Jun Liu, Qiu Cui, Yingang Feng* (2019) Solution structure of a unicellular microalgae-derived translationally controlled tumor protein revealed both conserved features and structural diversity.Arch. Biochem. Biophys.665(1):23-29.
6. Shiyue Liu†, Ya-Jun Liu†, Yingang Feng, Bin Li and Qiu Cui* (2019) Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization.Biotechnol. Biofuels12:35.
7. Chao Chen, Hongwu Yang, Jinsong Xuan, Qiu Cui, Yingang Feng* (2019) Resonance assignments of a cellulosomal double-dockerin from Clostridium thermocellum. Biomol. NMR Assign. 13(1):97-101.
所获专利(近五年)
1.采用氧气处理方法的木质纤维素全细胞糖化技术;申请号CN202210645875.1;崔球,刘亚君,陈朝阳,宋晓金
2.原位分析微生物单菌落的激光电喷雾质谱装置;申请号202011006611.9;崔球,刘欢,宋晓金,王森,王卓君,蓝传增;2021.5.18授权
3.原位分析微生物菌落中角鲨烯含量的方法;申请号202011006613.8;宋晓金,刘欢,崔球,王森,张慧丹,万伟建,蓝传增;2021.8.17授权
4. PET降解生物催化剂及其应用;申请号202010013024.6;崔球,刘亚君,颜飞,韦韧;2021.12.31授权
5.富含均衡不饱和脂肪酸的橄榄油衍生物的制备及应用;申请号201910519311.1;崔球,邓煜,宋晓金,刘婷;2022.3.15授权
6.改善鸡蛋蛋壳质量的饲料及养殖方法;申请号201910366029.4;崔球,刘冰,宋晓金,程琦,周晓峰,王彦超;2021.11.19授权
7.一种低成本,高生物量和高蛋白量的蛋白核小球藻培养方法;申请号201811569914.4;崔球,肖艳,颜飞,祁宽;2022.4.15授权
8.用于生产高DHA含量鸡蛋的饲料添加剂及应用;申请号201811285475.4;崔球,宋晓金,刘亚君,蓝传僧,王森
9.用于生产适合婴幼儿的DHA-ARA鸡蛋的营养强化剂及应用;申请号201811282278.7;崔球,初欢欢,宋晓金,刘亚君,张慧丹;2021.4.13授权
10.一种无抗生物鸡饲料的制备方法;申请号201811277537.7;崔球,刘亚君,刘世岳,李仁民
11.生物饲料用促消化复合菌剂及其应用;发明专利号201811276681.9;崔球,初欢欢,刘亚君,刘世岳,李仁民;2020.10.20授权
12.采用木质纤维素生产纤维寡糖的方法;发明专利号201810985861.8;崔球,刘亚君,李仁民,冯银刚;2021.1.1授权
13.用于催化木质纤维素糖化的全菌酶制剂;申请号201810939329.2;崔球,刘亚君,刘世岳,李仁民,祁宽,冯银刚;2021.4.13授权
14.提高木质纤维素糖化效率的全菌糖化方法;发明专利号201810939479.3;崔球,刘亚君,刘世岳,冯银刚;2020.10.20授权
15.采用木质纤维素制备角鲨烯的方法;申请号201810939517.5;崔球,刘亚君,宋晓金;2021.4.27授权
16.采用木质纤维素制备虾青素的方法;申请号201810939518.X;崔球,刘亚君,宋晓金;2021.4.27授权
17.用于催化木质纤维素糖化的纤维小体酶制剂;申请号201810939532.X;崔球,刘亚君,李仁民,刘世岳,祁宽,冯银刚;2021.4.13授权
18.采用木质纤维素培养微藻的方法;申请号201810939294.2;崔球,刘亚君,祁宽,宋晓金;2021.9.28授权
19.采用木质纤维素生产富含多不饱和脂肪酸油脂的方法;申请号201810939296.1;崔球,刘亚君,李仁民,宋晓金,张慧丹;2022.04.15
20.采用木质纤维素制备乙醇的方法;申请号201810939170.4;崔球,刘亚君,李滨,冯银刚;2022.04.15授权
21.采用木质纤维素制备葡萄糖酸钠的方法;申请号201810939181.2;崔球,刘亚君,祁宽,冯银刚;2021.4.27授权
22.采用木质纤维素制备DHA的方法;申请号201810939182.7;崔球,刘亚君,刘世岳,宋晓金;2021.10.1授权
23.木质纤维素一锅法生物转化方法;发明专利号201810939277.9;崔球,刘亚君,李滨,冯银刚;2020.10.20授权
技术转移转化
1.高效基因转化仪,青岛百奥新创生物技术有限公司
2.高通量菌株选育系统,青岛百奥新创生物技术有限公司
3. DHA单细胞油脂生产技术,青岛琅琊台集团
4. ARA单细胞油脂生产技术,青岛琅琊台集团
5.非粮生物质高效转化联产单细胞蛋白及油脂,山东寿光巨能金玉米开发有限公司
6.微生物油脂高效发酵生产技术,河南新拓洋生物工程有限公司
7.盐湖卤虫营养强化的单细胞蛋白饲料生产技术,山西南风化工集团股份有限公司
软件著作权
1.高通量基因转化系统控制平台,2023SR0808352
2.青能所代谢组学视觉预处理平台,2023SR0752423
3.青能云数据可视化分析平台,2023SR0752424
4.智能生物实验室深度学习视觉标注系统,2023SR0988017
5.智能实验室DNA序列编辑系统,2023SR0989011
6.智能液体处理系统控制平台,2023SR0808353
7.自动化生物实验室机器人集群控制平台,2023SR0808351
获奖情况
1. 2023.11,高产DHA海洋微藻的菌株优选及清洁生产示范,中国食品工业协会科学技术奖,排名第三
2. 2022.07,秸秆木质纤维清洁高效预处理关键技术及应用,中国石油和化工联合会技术发明三等奖,排名第三
3. 2017.03,高产ARA高产高山被孢霉的菌株选育及清洁产生示范,青岛市科技进步三等奖,排名第一
4. 2016.01,高产DHA海洋微藻的菌株选育及清洁生产示范项目,山东省科学技术奖,排名第一